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June 06-07, 2019 | London, UK
2
nd
International Conference on
Tissue Science and Molecular Biology,
Stem Cells & Separation Techniques
Joint Event
Biomedical Research (An International Journal of Medical Sciences) | ISSN: 0976-1683 Volume 30
Total tissue regeneration in necrosed diabetic foot with new biomedical strategy
Debora Nino-Laffont
Iberoamerican Institute of Bioregenerative Medicine (IBIOMER), Mexico
T
he present exposes a medic protocol to treat
endothelial dysfunction and clinical and subclinical
inflammation, whose therapeutic approach is based
on the principle of extended mitochondrial hormesis,
as previous physiological conditioning
ex vivo
/
in vivo
,
to promote the angiogenic power of autologous stem
cell transplantation mesenchymal (MSCs) from bone
marrow and whithout culture. Mitohormesis behaves
as a subtle extrinsic disturbance that triggers a series of
nuclear signaling events with biochemical and metabolic
changes that induce a cytoprotective state, which can
be obtained with oxygen-Ozone (O
2
/O
3
) applications at
low doses, through different routes of administration for
15 continuous days before and 15 continuous days after
autologous transplant MSCs, producing a cytoprotective,
anti-oxidant and anti-inflammatory microenvironment that
synergizes the angiogenic power and immunoregulatory
of MSCs. Diabetes Mellitus (DM) is an inflammatory
pathology, where there is a chronic hyperglycemic state
alters the molecular/cellular structures of vessels and
nerves, generating hyperplasia, endothelial dysfunction
and inflammation with cellular hypoxia. It has as a
complication diabetic foot (DF) in 10-15% and that it occurs
with ulceration, infection and destruction of deep tissues of
the lower extremity. I present a clinical case of male of 47
years, with Dx of DM of 18 years and with DF of 3 years of
evolution that degenerated in a state of necrosis, DF grade
III/IV, indicating partial amputation that rejected to be
submitted to combined protocol O
2
/O
3
-MSCs, giving results
of total tissue regeneration, 30 days after the first autologous
MSC with previous
ex vivo
/
in vivo
physiological conditioning,
which was evidenced clinically by signs of trophism and
functional recovery. After 12 months, the cycle was repeated
and there was improvement in the protective sensibility of
both lower limbs (Semmens-Weinstein monofilament) and
improvement of erectile dysfunction (IIEF-5), as an expression
of microvascular regeneration and systemic and autonomic
nervous connections. Prospective studies are carried out
evaluating clinical correlation of the angiogenic power with
the use of the combined O
2
/O
3
-MSCs protocol and if the
periods between cycles could be shortened.
e
:
deboraninolaffont@gmail.comBiomed Res, Volume 30
ISSN: 0976-1683