23 / 27
allied
academies
Page 60
June 06-07, 2019 | London, UK
2
nd
International Conference on
Tissue Science and Molecular Biology,
Stem Cells & Separation Techniques
Joint Event
Biomedical Research (An International Journal of Medical Sciences) | ISSN: 0976-1683 Volume 30
Liver tissues regenerated from human tooth treats liver failure of rat cirrhosis
model and swine NASH model
Ken Yaegaki
Nippon Dental University, Japan
C
adaveric or live-donor liver transplant is only the
treatment for severe liver condition. However, the
number of transplantations is very limited because of fewer
available organs than number of the patients on the waiting
list. The liver regeneration might be one of the alternatives.
Several clinical studies employed mesenchymal stem cells
from blood, adipose tissue or others to transplant without
differentiating the cells. However, transplantation of these
cells canonly slowdeclineof hepatic function, but they cannot
treat the conditions of the liver. The objective of adult stem
cell transplantationsmight be to launch “bridge to transplant”
strategy rather than treating liver condition. We have shown
that human dental pulp stem cell demonstrates huge
potential to treat lethal liver conditions. We have previously
reported we treated the biliary liver cirrhosis and acute liver
injury in nude rats with transplanting the regenerated liver
tissues originated from human dental pulp. One of the most
prevailing liver conditions is non-alcoholic steatohepatitis
(NASH). Hence the objectives of the research are to evaluate
the clinical possibility of our transplantation protocols using
swinemodelofprogressive liverfailuredevelopedfromNASH.
After four weeks of transplantation of hepatocytes described
from human tooth into the spleen of 6 swine with the failure
under immune suppression, we found the secondary liver in
the spleen was produced, as well the regenerated liver was
produced using the original liver as scaffold. Biliary ducts
are reproduced with human tissues only 4 weeks after the
transplantation. Serumalbumin level recovered from1.5 g/dL
to over 3.0 g/dL. HPT, choline esterase, collagen type IV, ALT
and others have been dramatically improved. But any of the
positive control has shown no change. Following above we
also treated rat cirrhosis model.
e
:
michiyo-y@tky.ndu.ac.jpBiomed Res, Volume 30
ISSN: 0976-1683