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June 06-07, 2019 | London, UK
2nd International Conference on
Tissue Science and Molecular Biology,
Stem Cells & Separation Techniques
Joint Event
Biomedical Research (An International Journal of Medical Sciences) | ISSN: 0976-1683 Volume 30
Mesenchymal stem cells-based therapy for glaucoma through regeneration of the
trabecular meshwork
Christian Tebid Tebid
Centre de recherche Hôpital Maisonneuve-Rosemont (CRHMR), Canada
In open angle glaucoma, dysfunction of the trabecular
meshwork (TM) leads to elevated intraocular pressure
(IOP) and concomitant optic nerve damage. Consequently,
regeneration of the TM cells may represent an effective
therapeutic option formany cases of glaucoma.Wepreviously
demonstrated the regenerative effects of mesenchymal stem
cells conditioned media (MSC-CM) in tissue regeneration in
laser damaged TM. This process led to a decrease in IOP in a
ratmodel of glaucoma. Thismechanism is depended solely on
macrophage recruitment. In this study, we have investigated
the mechanism of MSC-CM educated macrophages on tissue
regeneration. To better understand the role of macrophages
in the TM regeneration process, rats were pretreated with
clodronate liposomes resulting ina reduction in thenumber of
macrophages within the damaged TM. This culminated in the
attenuation of the effect of MSC-CM on the IOP. In addition,
reintroduction of in vitro MSC-CM educated macrophages
into macrophage depleted eyes restored the healing effect.
Furthermore, to elucidate the mechanistic basis of MSC-CM
educatedmacrophages-mediateddecreaseinIOP,weinjected
the supernatant from MSC-CM educated macrophages into
glaucomatous eyes. Surprisingly, this resulted in a decrease in
the IOP, thus indicating that MSC-CM educated macrophages
mediate TM regeneration and a decrease in IOP through
paracrine factor secretion. In addition, we have identified one
factor which we call RPF1 (Regenerative paracrine factor 1)
produced by MSC-CM educated macrophages as a potent
mediator of this regenerative effect. This finding provides a
novel cellular therapeutic approach for glaucoma treatment.
e:
Biomed Res, Volume 30
ISSN: 0976-1683