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June 06-07, 2019 | London, UK
2nd International Conference on
Tissue Science and Molecular Biology,
Stem Cells & Separation Techniques
Joint Event
Biomedical Research (An International Journal of Medical Sciences) | ISSN: 0976-1683 Volume 30
Advances in separation science and bioinformatics for analysing glycosylation in
manufacturing biologics
Pauline M Rudd
NIBRT Dublin, Ireland
Controlling glycosylation is a major issue for ensuring te
safety and efficacy of Biologics. Traditional challenges for
glycan analysis can be addressed by a range of technologies
that are automated, high throughput, sensitive and
quantitative. To give a more complete detailed analysis
of intact glycoforms a combination of technologies such
as LC/MS/CE are required. These enable the analysis of
intact glycoproteins, glycopeptides, released glycans and
glycolipid head groups. The bottleneck now lies in data
interpretation so this talk focuses on the application of four
new software programmes: (i) GlycopeptideGraphMS (for the
detailed identification of glycopeptides which is data base and
platform independent) (ii) GlycoStore (an international resource
including experimental glycan data bases and metadata) (iii)
Glycoanalyser (software to aid interpretation of exoglycosidase
array digestions) and (iv) MAGMap (a programme giving a
confidencescoretoanassignmentbasedonmultipleattributes).
e:
Biomed Res, Volume 30
ISSN: 0976-1683