tissue-science-molecular-biology-2019-posters

20 / 27

allied
academies
Page 57
Notes:
June 06-07, 2019 | London, UK
2nd International Conference on
Tissue Science and Molecular Biology,
Stem Cells & Separation Techniques
Joint Event
Biomedical Research (An International Journal of Medical Sciences) | ISSN: 0976-1683 Volume 30
From tissues to cells and back: Microfluidics-enabled solutions for parallel and
time-resolved multi-parameter characterization of individual cells across a
population
Jens Ducree
Dublin City University, Ireland
Aset of techniques to separate, analyse and manipulate
single cells provided from a suspension, e.g. as obtained
from tissue samples will be presented. Taking advantage of
effects specific to microstructures or microconfinement, the
cellular response of exposure to a sequence of stimuli and
stains can be monitored for all cells within a cohort in parallel
and even in real time. Cells of interest may be selected and
forwarded to further analysis, e.g. for content screening or
propagation. The presentedmicrofluidic systems have proven
to work with label-based methods, typically for validation, as
wellasdirectphotonic“fingerprinting”andtoresolvevaluable
detail on population statistics and dynamics way beyond
averages based on end-point assays. Examples are studies
on glycosylation, secretion and inflammation, for instance to
relate affected tissue to the onset of cardiovascular disease.
e:
Biomed Res, Volume 30
ISSN: 0976-1683