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June 06-07, 2019 | London, UK
2
nd
International Conference on
Tissue Science and Molecular Biology,
Stem Cells & Separation Techniques
Joint Event
Biomedical Research (An International Journal of Medical Sciences) | ISSN: 0976-1683 Volume 30
Maintaining physiological fidelity during
in vitro
stem cell culture and expansion; a
role for oxygen control
Nicholas R Forsyth
Keele University, UK
H
ypoxia or physiological normoxia, plays a key role in
determining stem cell behaviour in the
in vivo
niche. In
spite of this little attention is payed to the role of reduced
oxygen levels during
in vitro
culture generating a risk of
forced paradigm and artefactual norms. Bone marrow-
derived human mesenchymal stem cells (hMSC), due to the
sinusoidal blood vessel architecture found within their niche,
are particularly vulnerable to oxygen tension fluctuations.
We and others, have now described fundamental, artefactual,
alterations in hMSC biology as a consequence of air oxygen
exposure. These include reduced colony forming unit-
fibroblastic isolation; dysregulated epigenome, transcriptome
and proteome; altered biochemical volatile footprints
during culture; and counter intuitive alterations in reactive
oxygen species management. This lecture will discuss
the fundamental biology underpinning these biological
differences, their potential impact on regenerative medicine
and what we can do to transform biological understanding
into therapeutic application.
e
:
n.r.forsyth@keele.ac.ukBiomed Res, Volume 30
ISSN: 0976-1683