nutrition-world-2017-scientifictracks-abstracts

11 / 25

alliedacademies
Insights Nutr Metab 2017
Volume 1 Issue 3 Nutrition World 2017
Notes:
Page 29
September 11-12, 2017 Edinburgh, Scotland
15 th World Congress on
Advances in Nutrition, Food Science & Technology
Immunomodulating effects of the β-glucan
from Pleurotus cornucopiae mushroom on
macrophage actions
Ken-ichiro Minato1, Lisa C Laan2, Irma van Die2 and Masashi Mizuno3
1Meijo University, Japan
2VU University Medical Center, Netherlands
3Kobe University, Japan
Many edible mushrooms have become attractive
as health food and as source materials for
immunomodulators. Recently, the polysaccharide (PCPS)
from Pleurotus citrinopileatus mushroom has been
identified as a β-glucan which activates dendritic cells
(DCs) by upregulation of the secretion or expression
of many pro-inflammatory mediators. Moreover, it has
been shown that the PCPS has the capacity to activate
the cells via multiple pathways. In this study, we set
out to investigate the immune modulating properties of
the PCPS using macrophage-like cells derived from a
THP-1 cell line as well as DCs. The PCPS stimulated
the THP-1 macrophages to secrete significant levels of
TNF. Moreover, the mRNA expression of TNF and IL-
1β were significantly enhanced by the PCPS treatment.
However, the glucan did not induce to express both IL-
12 and IL-10 mRNA in the macrophages. Next, in vivo
experiments, the P. cornucopiae extract (containing
mainly PCPS) treatment against BALB/c mice showed
significant increases in TNF and IL-1β mRNA expressions
in the peritoneal macrophages of them. These results
suggested that the PCPS could induce pro-inflammatory
action in an innate immune response. Meanwhile, the
PCPS-treatment did not show any influence on an
expression of IFNγ mRNA in the lymphocytes of the mice
spleen despite it inhibited an expression of IL-4, an anti-
inflammatory cytokine, mRNA in this study. Moreover,
interestingly, regarding the influence of the PCPS on
macrophage differentiation, the glucan suppressed the
secretion of pro-inflammatory cytokines, such as TNF and
IL-6, from differentiated macrophages, suggesting that the
PCPS could promote monocyte to differentiate into M2
macrophage. These findings suggested that this edible
mushroom, P. cornucopiae, could pleiotropically regulate
macrophage activities by the β-glucan.
Biography
Ken-ichiroMinato is working as anAssociate Professor at Department ofApplied
Biological Chemistry, Meijo University, Japan. Ken-ichiro Minato devotes to
find a suitable functional food which could maintain our immune system. His
own research interest has been how food factors, such as polysaccharides
and polyphenols, act as an immunomodulator for monocyte, macrophages and
dendritic cells in an innate immune system. His current targets are both pro-
and anti-inflammatory effects of β-glucan in edible mushrooms on activities of
those innate immunocompetent cells. Another his interest is a differentiation
of macrophage toward M1/M2 and their activities. Macrophages develop
from hematopoietic stem cells through common myeloid progenitors in the
bone marrow, and repopulate in peripheral tissues. Currently it is thought
that macrophages can be classified into several different phenotypes, based
on their reactions to different microenvironments. The heterogeneity of
undifferentiated circulating monocytes may affect their polarization once they
arrive in inflamed tissues. He hopes to find a suitable functional food, which
could prevent inflammatory diseases.
Ken-ichiro Minato et al., Insights Nutr Metab 2017