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Insights Nutr Metab 2017
Volume 1 Issue 3 Nutrition World 2017
Notes:
Page 30
September 11-12, 2017 Edinburgh, Scotland
15 th World Congress on
Advances in Nutrition, Food Science & Technology
Comparison of gut microbiota in hens of the
crosses Hisex Brown and Lohmann Brown
Michael N Romanov1,2, Darren K Griffin1,2, Aleksandr N Panin 2,
Ivan I Kochish2, Vladimir I Smolensky2, Georgy Yu Laptev3, Ilya N
Nikonov3 and Larisa A Ilyina3
1University of Kent, UK
2Moscow StateAcademy of Veterinary Medicine and Biotechnology, Russia
3Biotrof+ Limited, Russia
C hanges in the composition of the intestinal microbio-
ta in the cecum of poultry could have a direct impact
on the quality and safety of poultry products. This study
presents the results of comparative molecular genetic
analysis of the cecal microbiocoenoses in laying hens
of two commercial crosses, Hisex Brown and Lohmann
Brown, during ontogeny. According to the analysis of over-
all taxonomic representation, more than 70% phylotypes
determined can be attributed to three: phyla, Firmicutes,
Bacteroidetes, and Proteobacteria. Less represented
were Actinobacteria, Tenericutes and Fusobacteria, and
the presence of significant amounts of unidentified bacte-
ria was also revealed. During ontogenesis, birds exhibited
marked changes in the ratio of the number of phylotypes
and taxonomic groups of the intestinal microbiota. Chick-
ens of both crosses went through several stages in the de-
velopment of microbial communities, including a stabiliza-
tion period at the age of 20 to 40 weeks, as evidenced by
the biodiversity assessment using ecological indexes. The
stabilization period was characterised with a significant
increase in representatives of class Clostridia involved
in the metabolism of carbohydrates, and in bacteria with
high antagonistic properties (genera Lactobacillus and
Bacillus). There was also a significant reduction of num-
ber of opportunistic and pathogenic taxa, such as fami-
lies Campylobacteraceae and Enterobacteriaceae, order
Pseudomonadales, and phylum Tenericutes. Despite the
similar conditions of housing and feeding, the Lohmann
Brown hens had a maximum level of representatives
of the normal flora observed by 40 weeks of age. This
probably determines a smaller number of pathogens like
Staphylococcus, family Campylobacteraceae, and phyla
Tenericutes and Fusobacteria found by 40 to 60 weeks
of age and greater stability of intestinal microbiocoenosis
in the Lohmann Brown birds as compared with the Hisex
Brown chickens.
This research is supported by a grant of the Government
of Russian Federation, Contract No. 14.W03.31.0013
Biography
Michael N Romanov has his expertise in avian genetics and genomics includ-
ing participation in a number of national and international research projects in
the areas of avian genetic diversity, gene/genome mapping, candidate genes
evaluation, and comparative genomics. After years of experience in studies
and teaching in both research and education institutions, he recently began
leading on a project sponsored by the Government of Russian Federation.
The project is aimed at developing state-of-the-art biotechnologies to assess
gene expression in relation to performance and disease resistance in poultry
industry, and will be done in collaboration with the Moscow State Academy of
Veterinary Medicine and Biotechnology in 2017–2019.
Michael N Romanov et al., Insights Nutr Metab 2017