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Insights Nutr Metab 2017
Volume 1 Issue 3 Nutrition World 2017
Notes:
Page 39
September 11-12, 2017 Edinburgh, Scotland
15 th World Congress on
Advances in Nutrition, Food Science & Technology
Determination of microbiocoenosis in
the intestine of the Hisex Brown hens in
ontogenesis using T-RFLP method
Michael N Romanov1,2, Georgy Yu Laptev3, Ilya N Nikonov3, Larisa A
Ilyina3 , Natalia I Novikova3, Olga V Barkova4, Darren K Griffin 1, Ivan
I Kochish2, Vladimir I Smolensky2, Aleksandr N Panin and Mikhail N
Shaposhnikov
1University of Kent, Canterbury, UK
2Moscow State Academy of Veterinary Medicine and Biotechnology, Russia
3BIOTROF+ Ltd, Russia
4Russian Research Institute of Farm Animal Genetics and Breeding, Russia
Microbiocoenosis in the gastrointestinal tract, especially
in the ceca, play an important part in life processes
of poultry. Identification of the structure and taxonomic
composition of microorganisms in the cecum using
molecular genetic methods serves as a crucial approach
in understanding how a cecal microbiota interplays
with the chicken organism during ontogenesis. For this
purpose, we studied an intestinal bacterial community
composition in the ceca of the Hisex Brown laying hens
at age of 40, 155 and 315 days using T-RFLP and RT-
PCR. In the chickens studied, development of the cecal
microbial communities, changes in their content, and
appearance of new microorganisms occurred in the
ontogeny. A broader spectrum of bacteria was found in
40- and 155-day-old birds (221±11 and 258±9 phylotypes,
respectively) as compared with 315-day-old laying hens
(178±8 phylotypes). Also, 315-day-old birds showed the
least content of unidentified phylotypes. In the ceca of
adult hens, there was a change in the dominant microbial
taxonomic groups including a higher proportion of acid-
utilising bacteria of the class Negativicutes and cellulolytic
bacteria of the class Clostridia, with a lower content of the
classes Bifidobacteriales and Bacillales. Lactobacteria
(order Lactobacillales) showed a greater content in
315-day-old laying hens (33.15±1.05%) as compared
with 40- and 155-day-old birds (5.13±0.23% and 24.58 ±
0.86%, respectively). The variety and number of bacteria
in the ceca conventionally attributed to various pathogens
of poultry diseases, including the genera Enterobacter,
Pantoea, Listeria, Acinetobacter and Mycoplasma,
families Campylobacteraceae and Pasteurellaceae, and
phylum Fusobacteria, increased with the age of birds.
Thus, during molecular genetic studies, the species
composition and dynamics of the microbiocoenosis in the
cecum of the Hisex Brown laying hens was determined as
related to their ontogeny.
This research is supported by a grant of the Government
of Russian Federation, Contract No. 14.W03.31.0013
Biography
Michael N Romanov has his expertise in avian genetics and genomics
including participation in many national and international research projects in
the areas of avian genetic diversity, gene/genome mapping, candidate genes
evaluation, and comparative genomics. After years of experience in studies
and teaching in both research and education institutions, he recently began
leading on a project sponsored by the Government of Russian Federation.
The project is aimed at developing state-of-the-art biotechnologies to assess
gene expression in relation to performance and disease resistance in poultry
industry, and will be done in collaboration with the Moscow State Academy of
Veterinary Medicine and Biotechnology in 2017–2019.
Michael N Romanov et al., Insights Nutr Metab 2017