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June 06-07, 2019 | London, UK
2nd International Conference on
Tissue Science and Molecular Biology,
Stem Cells & Separation Techniques
Joint Event
Biomedical Research (An International Journal of Medical Sciences) | ISSN: 0976-1683 Volume 30
Proliferation of human endometrial stem cells are experimented by C0-culture of
mouse fallopian stem cells
Shokria Ehsani
Ghalib University, Afghanistan
Introduction: Endometrial stem cells like other adult stem
cells are rare undifferentiated cells present in most adult
tissue. Concerning to the valuable application of human
endometrial stem cells in clinic and tissue engineering, the
proliferation of human endometrial stem cells is going to be
one of the important issues among researcher. Recently, it has
been reported that growth factors like leukemia inhibitory
factor in medium culture of adult stem cells is necessary to
maintain MSC self-renewal and undifferentiated. However,
the effect of leukemia inhibitory factor on proliferation and
pluripotency of human endometrial stem cells, which have
very important, has not been explored. In this study we tried
to investigate the effects of LIF on ESCs proliferation and
pluripotency.
Materials and Methods: The endometrial cells were collected
from the uterus of hysterectomies samples. Then the isolated
cells were cultured in the DMEM+F12 with LIF. In experimental
group study 10 ng/ml LIF was added to culture and at the
end of forth subculture and after treatment with LIF the
CD90 positive cells were evaluated using flow cytometry.
The proliferation rate of both experimental study and control
group using MTT assay were done. The expression of Nanog,
Oct4, PCNA and LIFr genes was evaluated using real time-PCR
in high proliferation are (LIF treated) group.
Results: The proliferation rate of treated and control groups
were 1/61 ± 0/06 and 1/1 ± 0/01. The rate of CD90 positive
cells before treatment with LIF was %94 (P<0.05) and after
treatment was %98 (P<0.05). The expression rate of all target
genes to housekeeping was higher in LIF treated group than
other group (P<0.05).
Conclusion: 10ng/ml LIF inmediumculturehas a great impact
on proliferation and pluripotency of human endometrial stem
cells LIF also increased the CD90 positive endometrial stem
cells and the expression of Oct4, Nanog, PCNA and LIFr. Co-
cultured groups have no significance effect on proliferation of
endometrial stem cells
Speaker Biography
Shokria Ehsani is a head of anatomy department in Ghalib University,
Afghanistan and she has many publications in the international journals.
e:
Shokria Ehsani, Biomed Res, Volume 30
ISSN: 0976-1683