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June 06-07, 2019 | London, UK
2
nd
International Conference on
Tissue Science and Molecular Biology,
Stem Cells & Separation Techniques
Joint Event
Biomedical Research (An International Journal of Medical Sciences) | ISSN: 0976-1683 Volume 30
Spire: A new Rab27a-effector characterizing Rab27a interaction-sites within spire
Noura Alzahofi
University of Nottingham, UK
R
ab27a is a small GTPase and a member of the RAS
oncogene family. Rab27a governs different Kinds of
intracellular trafficking through interaction with distinct
effectors. The extent of known Rab effectors has contributed
to enlightening the molecular mechanism and dysfunctions
that lead to a variety of human diseases. Recently, we identify
spire, an actin nucleation protein, as a new Rab-27a effector.
Spire interacts with formin-1 (an actin elongation factor) to
nucleate linear actin-filaments that are used as a track for
myosin to transport intracellular cargo, includingmelanosome
in skin melanocytes. Using melanosome distribution as an
indicator, in modified-nanoscale pulldowns assay, we found
that spire is able to interact with Rab-27a on the cytoplasmic
face of themelanosome via its C-terminal membrane- binding
region. In addition, the results highlight a crucial role of Spire-
Box domain in this interaction. Interestingly, a point mutation
within Spire-Box (K419W) blocked the said interaction. This
mutation corresponding to that of R35W in melanophilin/
Slac2 (Rab27a-effector) that causes Griscelli syndrome type-3
in humans.
Speaker Biography
Noura Alzahofi is currently pursuing her PhD in the University of
Nottingham, UK. She has few publications in international journals.
e:
Noura.Alzahofi@nottingham.ac.ukNoura Alzahofi
, Biomed Res, Volume 30
ISSN: 0976-1683