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Biomedical Research
|
Volume 29
Page 57
allied
academies
CARDIOLOGY AND CARDIOVASCULAR MEDICINE
STEM CELLS AND REGENERATIVE MEDICINE
&
International Conference on
International Conference on
J u n e 1 8 - 1 9 , 2 0 1 8 | O s a k a , J a p a n
Joint Event on
INVESTIGATING THE CELLULAR DYNAMICS OF ORGANS
DEVELOPMENT AND CANCER USING 3D IMAGING
Anne Rios
Princess Maxima Center, Netherlands
I
n collaboration with her colleague Nai Yang Fu, I implemented a novel 3D-imaging approach (with 3D glasses) to perform
innovative multicoloured lineage tracing studies to follow the development and fate of mammary stem cells (MaSC) and
descendant progenitor cells
in vivo
in entire mammary gland. As stem cells divide they produce clones of cells; using
this imaging technique the fate of these individual clones could be tracked throughout various stages of mammary gland
development, including puberty, pregnancy and normal adult homeostasis. This work provided the first
in vivo
evidence for
the existence of bipotent MaSCs, which give rise to the two cell lineages that constitute the mammary ducts, the luminal
and the myoepithelial cells, as well as the presence of distinct long-lived unipotent progenitor cells. The cellular dynamics
observed at different developmental stages support a model in which both stem and progenitor cells drive morphogenesis
during puberty, whereas bipotent MaSCs coordinate ductal homeostasis and remodelling of the adult mouse gland (Nature
2014, Nature Comm. 2016, NCB 2017). We have now specialized this 3D technology to detect early aberrant cellular
behaviour in models of breast cancer and to visualise how cancerous cells, according to their cell-of-origin, exit normal
ductal homeostasis and survive to self-organise into a solid tumour.
A.C.Rios@prinsesmaximacentrum.nlBiomed Res 2018, Volume 29 | DOI: 10.4066/biomedicalresearch-C2-006