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Biomedical Research
|
Volume 29
Page 54
Note:
allied
academies
CARDIOLOGY AND CARDIOVASCULAR MEDICINE
STEM CELLS AND REGENERATIVE MEDICINE
&
International Conference on
International Conference on
J u n e 1 8 - 1 9 , 2 0 1 8 | O s a k a , J a p a n
Joint Event on
THERAPEUTIC POTENTIAL OF STEM SELLS AND ZINC ON
REDUCTION OF LIVER FIBROSIS
Sulaiman Shams
Abdul Wali Khan University, Pakistan
G
lobally 1 out of 40 people died due to chronic liver diseases. In case of liver failure, transplantation is the last available therapy
but due to lack of donor, graft rejection, operative damage and high cost making this therapy unsuccessful. Stem cells
therapies developed new ways to treat liver diseases, but due to oxidative stress at damage site causes poor MSCs proliferation
and engraftment. The aim of the current study was to explore the therapeutic potential of ZnSO
4
and MSCs on CCl
4
induce hepatic
toxicity. In the current study, CCl
4
(1µl/g) was injected intraperitoneally to female BALB/c mice, twice in a week up till 4 weeks to
induce liver damage. MSCs was isolated from femoral and tibial bone of Balb/C mice and were cultured for two weeks. These
cultured cells and ZnSO
4
both were induce separately as well as in combination in mice body. The mice were then classified into
5 groups: negative control, positive control, CCl
4
+MSC treated group, CCl
4
+ZnSO
4
treated group and CCl
4
+MSCs+ZnSO
4
treated
group. The morphological results showed that in contrast to only MSCs therapy, ZnSO
4
along with MSCs showed significant
therapeutic result on CCl
4
injured mice. Biochemically, serum ALT and total bilirubin level were found to be significantly decreased
in mice treated with ZnSO
4
and MSCs. Histopathological examination also revealed that both ZnSO
4
and MSCs have strong anti-
apoptotic effect on CCl
4
injured liver by decreasing the number of apoptotic hepatocytes in both ZnSO
4
and MSCs transplanted
mice. RT-PCR results at mRNA level also confirm a significant anti-fibrotic effect of ZnSO
4
and MSCs (in combination) transplanted
mice on fibrotic liver as evidenced show the down-regulation of apoptotic marker (Bax) and enhancing anti-apoptotic (Bcl-xl)
and hepatic marker (Albumin). Thus it is concluded that ZnSO
4
is a powerful antioxidant and have the ability to enhance the
proliferation rate of MSCs.
Sulaiman@awkum.edu.pkBiomed Res 2018, Volume 29 | DOI: 10.4066/biomedicalresearch-C2-006