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Biomedical Research
|
Volume 29
Page 55
Note:
allied
academies
CARDIOLOGY AND CARDIOVASCULAR MEDICINE
STEM CELLS AND REGENERATIVE MEDICINE
&
International Conference on
International Conference on
J u n e 1 8 - 1 9 , 2 0 1 8 | O s a k a , J a p a n
Joint Event on
EFFECTS OF ADULT MESENCHYMAL STEM CELLS IN THE
EPIDERMAL REGENERATION
Jee Woong Choi
Ajou University School of Medicine, South Korea
Objective:
To investigate the effects of the adult mesenchymal stem cells (AMSCs) on the epidermal regeneration,
in vitro
living
skin equivalents (LSEs) were reconstructed by co-culture of keratinocytes with each of the different cell types (fibroblasts, adipose-
derived stem cells (ADSCs), bone marrow mesenchymal stem cells (BMSCs)) as dermal matrix cells.
Method:
Characteristics of the epidermal structures together with keratinocyte growth, differentiation, and basement membrane
integrity were analyzed by H&E staining. Additionally, immunohistochemistry was used to study the expression patterns of
proteins related with wound healing, epidermal proliferation, differentiation, and basement membrane formation. The author also
performed high-throughput mRNA sequencing of three types of dermal matrix cells in order to clarify the causes of differences
in characteristics between cultured LSEs. The associated protein expression was evaluated by signal intensity scoring and image
analysis using immunofluorescence images.
Results:
Compared to fibroblast base LSEs, stem cell based LSEs showed similar appearances to normal skin. It can be assumed
that the stem cell based LSE is thicker and more real skin-like due to the increased genes that were grouped as EGF-like domain
cluster. Moreover, the basement membrane was clearly identified in the stem cell based LSE. Similar phenomenon was also seen
in integrin immunoflourescent staining. We also found that galectin-7 was strongly expressed in stem cell based LSEs which
seem to have good ability of keratinocyte differentiation. Activin A was increased in AMSCs in high-throughput mRNA sequencing
analysis, and was expressed largely in both epidermal and dermal layers in immunofluorescent staining.
Conclusion:
The proteins produced from the stem cells of the LSE affects the basal keratinocytes through paracrine effects, and
contribute to epidermal proliferation and keratinocyte migration. Especially, activin A may have an important role in wound healing
as a mediator in dermal-epidermal interaction.
dermaboy@gmail.comBiomed Res 2018, Volume 29 | DOI: 10.4066/biomedicalresearch-C2-006