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allied
academies
October 22-23, 2018 | Frankfurt, Germany
International Conference on
Robo t i c s a n d A u t oma t i o n
B iomater ial s and Nanomater ial s
Joint Event
&
Journal of Biomedical Research | Volume 29
Artificial 3D culture systems for T-cell expansion
Eduardo Perez del Río
CIBER-BBN, Spain
A
doptive cell therapy, which consists of the extraction,
manipulation, and administration of ex vivo generated
autologous T cells to patients, is an emerging alternative to
regular procedures in cancer treatment. Nevertheless, these
personalized treatments require laborious and expensive
laboratory procedures that should be alleviated to enable their
incorporation into the clinics. With the objective to improve
the ex vivo expansion of large amounts of specific T cells, we
used three-dimensional (3D) structures during their activation
with artificial antigen presenting cells, thus resembling the
natural environment of the secondary lymphoid organs. Thus,
the activation, proliferation, and differentiation of T cells were
analyzed when cultured in the presence of two 3D systems,
Matrigel and a 3D polystyrene scaffold, showing an increase
in cell proliferation compared to standard suspension systems.
Moreover, new synthetic biomaterials are being investigated
with the same purpose.
e:
eperez2@icmab.esRobotics & Biomaterials 2018, Volume 29
DOI: 10.4066/biomedicalresearch-C6-017