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allied
academies
October 22-23, 2018 | Frankfurt, Germany
International Conference on
Robo t i c s a n d A u t oma t i o n
B iomater ial s and Nanomater ial s
Joint Event
&
Journal of Biomedical Research | Volume 29
Utilization of seafood waste for potential biomedical applications
Jayashree Chakravarty
University of Massachusetts, USA
L
obster shell waste was used as the source of raw material
to produce chitin using biological treatment, which included
the use of co-cultures with a protease-producing bacterium,
either Bacillus megaterium NH21 or Serratia marcescens
db11, and an organic acid-producing bacterium Lactobacillus
plantarum. The optimal culture conditions, including co-
cultivation strategies and glucose concentrations, were
identified to improve the efficiency of deproteinization and
demineralization of lobster shells. The lobster shells were
also treated chemically for chitin extraction as comparison to
the bio-based treatments. Overall, the successive treatment
with a combination of S. marcescens db11 and L. plantarum
yielded the best co-removal of CaCO
3
and proteins from
lobster shell biomass, with total deproteinization of 87.19%
and total demineralization of 89.59%. Chitin membranes
were successfully prepared by dissolution of this microbially-
extracted chitin in ionic liquid 1-ethyl-3-methylimidazolium
acetate. The resulting materials were thoroughly characterized,
revealing that freeze-drying produced chitin membranes that
were highly porous. The drying methods and the concentration
of chitin used defined many of the membrane properties, such
as mechanical strength, porosity, and water absorbency. A
mathematical model was developed to correlate and predict
different polymer properties like tensile strength, which would
lead to the ability to tune the properties of the biomaterial.
Rayleigh’s method is often used to develop an expression in
the form of an exponential equation to show the functional
relationship for a variable that depends on other independent
variables. These chitinmembranes could potentially be used for
biomedical applications such as wound-dressing materials and
scaffolds in tissue engineering. The results from the proof-of-
concept study described here suggest that microbial treatment
may be an environmentally friendly alternative to the chemical
method of chitin extraction. This study provides a starting point
for the design and fabrication of a family of polysaccharide-
based sustainable materials with potentially broad applicability.
e:
jayachakravarty91@gmail.comRobotics & Biomaterials 2018, Volume 29
DOI: 10.4066/biomedicalresearch-C6-017