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Page 65
allied
academies
Joint Event
February 21-22, 2019 | Paris, France
Microbiology & Applied
Microbiology
2
nd
International Conference on
World Congress on
Wound Care, Tissue Repair
and Regenerative Medicine
&
Journal of Trauma and Critical Care | Volume 3
Isolation and characterization of Klebsiella pneumonia bacteriophage
Murad Ishnaiwer, Ameer Al sharif, Rawand Naji Ajlouni, Siham Al halaweh
and
Fawzi Al Razem
Palestine Polytechnic University, Palestine
K
lebsiella pneumonia
is a gram-negative, non-motile
bacteria that are found ubiquitously in nature. It frequently
causes human nosocomial infections especially in immune
compromised patients, leading to respiratory tract, urinary
tract and blood stream infections. Due to the extensive usage
of broad-spectrum antibiotics in hospitalized patients, the
incidence of multidrug resistance producing strains among
clinical isolates has been increasing. Consequently, this has
rekindled the interest in using phage therapy as a safe and
effective treatment for multidrug resistance pathogens. The
rapid ability of phages to lyse bacteria and their specificitymake
them effective alternative to antibiotics. Results demonstrated
a successful isolation of a
Klebsiella
bacteriophage isolated
from sewage water. The bacteriophage was able to host and
completely lyse the
Klebsiella
bacterium as a first case reported
in Palestine. The results were confirmed several times to
ensure consistency. It also gave positive results when spotted
on different
Klebsiella
strains. In addition, one-step growth
curve using a double layer plaque assay was performed to
determine the phage life cycle phases of infection. It showed
a latent period of about 3.5h, burst period of 10h and a burst
size of about 102.5×106 PFU /plaque, furthermore, SDS-PAGE
results revealed that four major bands have been detected for
phage structure proteins their size : 75KDa, 100KDa, 135KDa
and 180KDa. We believe the isolated phage can be used as
an effective and simple replacement to antibiotics used in the
treatment of
Klebsiella pneumonia
.
e:
mishnaiwer@ppu.eduJ Trauma Crit Care, Volume 3
DOI: 10.4066/2591-7358-C1-003