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allied
academies
Joint Event
February 21-22, 2019 | Paris, France
Microbiology & Applied
Microbiology
2
nd
International Conference on
World Congress on
Wound Care, Tissue Repair
and Regenerative Medicine
&
Journal of Trauma and Critical Care | Volume 3
Diversity of ligninolytic enzymes and their genes in the genus Ganoderma - application for
biodegradation of xenobiotic compounds
Giselle Torres Farrada
University of Havana, Belgium
W
hite Rot Fungi (WRF) and their ligninolytic enzymes are
considered promising biotechnological tools to remove
persistent organic pollutants from industrial waste waters
and polluted ecosystems. A high diversity within the genus
Ganoderma has been reported in Cuba; in spite of this, the
diversity of their ligninolytic enzymes, their genes and the
biotechnological potential of well adapted autochthonous
strains belonging to genus Ganoderma remains underexplored.
The objectives of this study were: To analyze the diversity of
ligninolytic enzymes and genes of Cuban native strains from
the genus Ganoderma and to evaluate their potential for
degradationoftextiledyesandpolycyclicaromatichydrocarbons
(PAHs). Thirteen WRF strains were isolated from decayed wood
in urban ecosystems in Havana and identified as Ganoderma
sp. using a multiplex ITS-based PCR-method. The strains were
cultured in SB-U medium with sugarcane molasses and the
ligninolytic enzymes activities as well as isozyme analyses were
measured on extracellular enzyme extracts. The diversity of
genes encoding laccases and peroxidases was determined
using a PCR and cloning approach with basidiomycete specific
primers. The results showed that Ganoderma sp. strains isolated
differed in their ligninolytic enzyme activities, isozymatic
profiles, laccase and peroxidase gene repertoires. A high
diversity of laccase genes was found among the strains; while
only one gene encoding manganese or versatile peroxidases
were detected. The translated laccases and peroxidases amino
acid sequences have not been described before. The strains
were able to significantly degrade textile dyes, naphthalene,
phenanthrene and fluorene. We found that the PAH oxidation
performed by their extracellular enzymes generated non-toxic
intermediate metabolites; the possible degradation pathways
of these PAHs were determined. These findings hold promises
for the development of a practical application for the treatment
of textile industry wastewaters, as well as for bioremediation
of polluted ecosystems by well-adapted native WRF strains.
e:
giselletf@fbio.uh.cuJ Trauma Crit Care, Volume 3
DOI: 10.4066/2591-7358-C1-003