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Joint Event
February 21-22, 2019 | Paris, France
Microbiology & Applied
Microbiology
2
nd
International Conference on
World Congress on
Wound Care, Tissue Repair
and Regenerative Medicine
&
Journal of Trauma and Critical Care | Volume 3
Identification of biosurfactant producing
Bacillus sp
. isolated from Sirri Island petroleum contaminated
soils
Mohammad Ghayyomi Jazeh, Atena Alirezaei Dizicheh, Ayat Nasrollahi Omran, Seyed Mansour Meybodi
and
Mansour Bayat
Islamic Azad University, Iran
B
iosurfactant are amphiphilic compounds produced by
microorganisms which either adhere to cell surfaces or
are excreted extracellularly in the growth medium. The aim of
this study was to isolate biosurfactant producing bacteria and
optimize the conditions like temperature, pH and using crude
oil, glucose and diesel as source for maximum biosurfactant
production. Samples were collected from 8 selected points of
oil contaminated soils in Sirri Island-Iran. Primary screening
tests including hemolytic activity, drop collapse technique
and oil spreading method were performed and species with
the best results were picked for complementary screening
tests like emulsification activity, foaming and surface tension
measurement. The isolated biosurfactant were identified
using TLC method. Totally, 81 bacteria species were isolated.
During primary and complementary screening tests, 29 species
showed hemolytic activity, 23 had drop collapsing ability and
18 species showed positive results in emulsification, foaming
and surface tension reduction. Finally, two
Bacillus sp
. were
found to be able to reduce surface tension less than 40mNm-
1. Two strains with a high amount of biosurfactant production
and emulsification ability were resulted from the present study.
According to the high potential of
Bacillus sp
. especially for
microbial enhanced oil recovery (MEOR) and bioremediation
of oil contamination we can hope that further study of the
isolates characteristics and looking for new local strains can
play an important role in their application in oil industry.
e:
mohammad_ghayyomi@yahoo.comJ Trauma Crit Care, Volume 3
DOI: 10.4066/2591-7358-C1-003