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June 06-07, 2019 | London, UK
2
nd
International Conference on
Tissue Science and Molecular Biology,
Stem Cells & Separation Techniques
Joint Event
Biomedical Research (An International Journal of Medical Sciences) | ISSN: 0976-1683 Volume 30
Processing of plant wastes using traditional and sophisticated high-pressure extraction
techniques
Martin Topiar, Kosovic E
and
Cerhova M
Institute of Chemical Process Fundamentals of the CAS, Czech Republic
W
orldwide overproduction in the food and cosmetics
industry creates an enormous amount of plant-
based wastes which are combusted or landfilled without
any additional use. Our project aims to utilize wastes
from winemaking industry (i.e. grape cane, grape marc
or grape seeds) for the extraction of valuable biologically
active substances, which can be used as by-products in
dietary supplements. Several extraction techniques including
maceration, Soxhlet extraction, ultrasound-assisted extraction
(UAE) and pressurized liquid extraction (PLE) were used to
obtain trans-resveratrol and ε-viniferine from grape cane
and grape marc, while grape seeds were treated by high-
temperature pressing, hexane maceration and supercritical
fluid extraction (SFE). The chemical composition of plant
isolates was determined using HPLC/MS and Folin-Ciocalteu
method. Experiments were performed via laboratory
equipment and newly developed multipurpose extraction
unit with vessels of volume 25 dm
3
and 20 dm
3
. The
chemical composition of isolates varied based on extraction
technique and its process parameters. High concentrations
of trans-resveratrol and ε-viniferine were identified in the
short-term maceration and Soxhlet isolates. Adopting of an
ultrasound probe into the process of maceration and Soxhlet
extraction led to a decrease in processing times from days
in terms of maceration and hours for Soxhlet extraction
to several minutes. On the top of that, concentrations of
target compounds were comparable with those obtained at
traditional maceration and Soxhlet extraction. Using of SFE
has substantial benefits on quality of grape seed oil, because
of low extraction temperatures (vs. high temperature
pressing) and absence of traces of any organic solvents (vs.
hexane extraction).
Speaker Biography
Martin Topiar has obtained his PhD degree at 2019 from the University
of Chemistry and Technology Prague with cooperation of the Institute
of Chemical Process Fundamentals of the CAS. He is focusing on the
SFE from plants with particular interest in a study of different types
of fractionation techniques. He has published 5 papers in reputed
journals (h-index 2, more than 20 citations) and presented his work in
many international conferences dealing with extraction techniques and
supercritical fluids utilization.
e:
topiar@icpf.cas.czMartin Topiar et al.
, Biomed Res, Volume 30
ISSN: 0976-1683