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June 06-07, 2019 | London, UK
2
nd
International Conference on
Tissue Science and Molecular Biology,
Stem Cells & Separation Techniques
Joint Event
Biomedical Research (An International Journal of Medical Sciences) | ISSN: 0976-1683 Volume 30
An automated perfusion bioreactor system for oxygen-controlled cultivation of 3D-cell
cultures
Jakob Schmid
Munich University of Applied Sciences, Germany
B
ioreactor systems are an important factor for the
successful cultivation of 3D-cell cultures, since they
are able to overcome limitations of static cell culture, by
providing proper nutrient and oxygen levels. A bioreactor,
which is suitable for the reproducible generation of tissue
engineered grafts (TEGs), as well as for the optimization
of culture conditions, has to meet several requirements,
such as built-in measurement instrumentation and control
technology, high throughput and high flexibility. In the
present study, a perfusion bioreactor system, which allows
for the oxygen-controlled cultivation of up to four TEGs in
independently operating bioreactors, was designed and
manufactured using rapid prototyping technologies. A
uniform flow distribution in the developedmicrobioreactor
was shown using computational fluid dynamics. The
integrated measurement instrumentation and control
technology allows for the cultivation at pre-set oxygen
levels. Furthermore, an automated cell-seeding protocol
ensures a homogeneous initial cell distribution and thus
a reproducible workflow. The developed microbioreactor
system opens up new possibilities in the field of tissue
engineering by enabling more reproducible experiments,
the investigation of optimal oxygen levels in 3D cell cultures
and by allowing for the generation of artificial tissue in an
oxygen-controlled environment.
Speaker Biography
Jakob Schmid is a PhD student at the center of applied tissue engineering
and regenerative medicine (CANTER) at the Munich University of Applied
Sciences. He earned his BSc in biotechnology at the Weihenstephan
UniversityofAppliedSciencesandhisMSc inpharmaceuticalbiotechnology
at the UlmUniversity. CANTER is a cooperative research lab of the Technical
University Munich, the Ludwig-Maximilians-University Munich and the
Munich University of Applied Sciences. The main focuses of CANTER are to
investigate methods of three-dimensional printing for tissue engineering
to open new possibilities and applications for tissue engineering research
and to characterize tissue and cell-cell interactions on a biophysical
level to increase knowledge about macromolecular functions in tissue.
e:
jakob.schmid0@hm.eduJakob Schmid
, Biomed Res, Volume 30
ISSN: 0976-1683