tissue-engineering-stem-cells-2019-posters-abstracts

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Journal of Biomedical Research | ISSN: 0976-1683 | Volume 30

March 14-15, 2019 | London, UK

T issue Engineer ing, Stem Cel ls and Regenerat ive Medicine

Cel l and Gene Therapy

World Congress on

International Conference on

Joint Event

Biomed Res, Volume 30

DOI: 10.4066/biomedicalresearch-C1-026

generation of germ cells from marrow derived mesenchymal stem cells in rat

Kuldeep Kumar, Kinsuk Das, Madhusoodan AP, Ajay Kumar, Purnima Singh, Tanmay Mondal

and

Sadhan Bag

Bioinformatics Technologies of India, India

erm cells must develop along distinct male or female

paths to produce the spermatozoa or oocyte required

for sexual reproduction. Mesenchymal stem cells (MSCs)

have the capacity to transdifferentiate into multilineage cells,

such as muscle of mesoderm, lung and liver of endoderm,

and brain and skin of ectoderm origin. Here we show that

the bone marrow derived stromal cells can transdifferentiate

to germ cell-like cells suggesting that bone marrow can be

a potential source of germ cells that could sustain sperm/

oocyte production. The bone marrow derived cells were

characterized using MSC specific markers (CD73, CD90 and

CD105) by molecular, immunocytochemistry and FACS analysis

and termed them rBM-MSC. These rBM-MSCs were subjected

to insitu differentiation into germ cells (GCs) with the help of

retinoic acid. Briefly, the semi confluent cells were treated with

low glucose DMEM medium with FBS and supplemented with

10-6 M RA for a period of 21 days. At the end of the treatment,

the existence of germ-like cells in the cultures were confirmed

by assessments of changes in cell morphology, expressions of

GC-specific marker genes i.e. Stella and Fregilis by RTPCR, FACS

and immunocytochemistry. Further, the differentiated cells also

expressed the known molecular markers of spermatogonial

stem cells viz c-kit, Stra8, dazl, Daz, Tex18 and Tp2. The study

revealed that the mesenchymal stem cells derived from bone

marrow have the potentiality to differentiate into germ cells

and opens the possibilities for use of these cells in reproductive

medicine.