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Journal of Biomedical Research | ISSN: 0976-1683 | Volume 30
March 14-15, 2019 | London, UK
T issue Engineer ing, Stem Cel ls and Regenerat ive Medicine
Cel l and Gene Therapy
World Congress on
International Conference on
&
Joint Event
In-vitro
pancreatic cancer cell imaging by indocyanine green based polymeric nanoprobes
Oguzhan Gunduz
1
, Zeynep R Ege
1
, Aydin Akan
2
, Faik N Oktar
1
, Chi C Lin
3
, Durdane S Kuruca
4
, Betul Karademir
1
, YesimM Sahin
5
and
Gokce
Erdemir
4
1
Marmara University, Turkey
2
Izmir Katip Celebi University, Turkey
3
Tunghai University, Boulevard, Taiwan
4
University of Istanbul, Turkey
5
Arel University, Turkey
I
ndocyanine green (ICG) provides an advantage in the imaging
of deep tumors as it can reach deeper location without
being absorbed in the upper layers of biological tissues in the
wavelengths, which named ‘therapeutic window’ in the tissue
engineering. Unfortunately, rapid elimination and short-term
stability in aqueous media limited its use as a fluorescence
probe for the early detection of cancerous tissue. In this study,
stabilization of ICG was performed by encapsulating it into the
biodegradable polymer composited with poly (L-lactic acid)
and poly(e-caprolactone) via a simple one-step multiaxial
electrospinning. Confocal microscopy was used to image the
encapsulated ICG within electro spun nanofibers and ICG
uptake by MIA PaCa-2 pancreatic cancer cells. The stability of
encapsulated ICG is demonstrated by the in vitro release profile
up to 21 days. These results suggest the potential of the ability
of internalization and accommodation of encapsulated ICG into
the pancreatic cell cytoplasm
e:
ucemogu@ucl.ac.ukBiomed Res, Volume 30
DOI: 10.4066/biomedicalresearch-C1-026