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Journal of Biomedical Research | ISSN: 0976-1683 | Volume 30
March 14-15, 2019 | London, UK
T issue Engineer ing, Stem Cel ls and Regenerat ive Medicine
Cel l and Gene Therapy
World Congress on
International Conference on
&
Joint Event
Label-free and non-invasive cell analysis in 3D tissue models and quality assurance of blood products
using Raman trapping microscopy
Schutze K
1
, Steinke M
2
, Kronstein-Wiedemann R
3
and
Tonn T
3
1
CellTool GmbH, Germany
2
University Hospital Würzburg, Germany
3
Medical Faculty Carl Gustav Carus, Germany
R
aman trapping microscopy (RTM) is a non-invasive, label-
free, highly sensitive analytical method for efficient and
fast identification and characterization of single cells in solution
or within 3D-tissue. Here, we present RTM as a novel tool for
gentle yet highly precise cell analysis in three independent
experiments, providing an overview about the large versatility
of this method.
We could show evidence that RTM is a suitable tool to
investigate if primary human tracheobronchial epithelial cells
used within an engineered 3D human airway mucosa tissue
model display tumour-specific characteristic. Furthermore, we
could observe cellular differentiation in 3D mucosa scaffolds
and monitor condition of blood products such as erythrocyte
and thrombocyte concentrates. Here, we could show that both
erythrocytes and thrombocytes have their own Raman profile.
In addition, change of Raman spectra with time was consistent
with routine quality control studies of decrease in platelet
activation capacity as well as with the correlation in metabolic
consumption. The identified Raman parameters could become
a quality feature for tissue models, but also for blood products
with regard to aging and functionality. First results also give
hint that RTM can identify bacterial contamination within
erythrocyte concentrates.
Increasingly there is a need to test functionality, integrity and
sterility of cell-based products during manufacture and of
the final product prior to transplantation. As Raman trapping
microscopy works label-free and requires less than 500 cells
for analysis it has the potential to become a standard for fast,
efficient and highly reliable quality control of any advanced
therapy medicinal product.
e:
k.schuetze@celltool.deBiomed Res, Volume 30
DOI: 10.4066/biomedicalresearch-C1-026