16 / 18
Page 46
Notes:
allied
academies
Journal of Biomedical Research | ISSN: 0976-1683 | Volume 30
March 14-15, 2019 | London, UK
T issue Engineer ing, Stem Cel ls and Regenerat ive Medicine
Cel l and Gene Therapy
World Congress on
International Conference on
&
Joint Event
In vitro
differentiation of mesenchymal stem cells from human umbilical cord Wharton’s jelly into
functioning hepatocytes
Mervat Dawood
Mansoura University, Egypt
Back ground:
The human umbilical cord (UC) is non-invasive,
primitive and abundant sources of mesenchymal stromal cells
(MSCs) that have increasingly. Liver disease is a major cause of
mortality and morbidity in Egypt. There are many inflammatory
liver conditions for which treatments are not effective and
often such patients will progress to end-stage liver disease and
require liver transplantation. To prevent progression to end-
stage liver disease, mesenchymal stromal cell (MSC) therapies
have been considered and shown to have potential in such liver
diseases.
Objectives:
The aim of our study was to investigate the
in vitro
differentiation of human umbilical cord Wharton’s jelly (HU-
MSC) into hepatocyte lineage.
Materials & methods:
Human umbilical cord Wharton’s jelly
(WJ) were separated by mixed explant & enzymatic method
by use of trypsin. The time required for the primary culture
range from 10-14 days. The isolated cells were characterized
for expression of MSC-specific markers such as CD73, CD90
and CD105 & CD45. Also cells were counted by automated cell
counter for stem cells (showing count, viability, cluster cells).
After passage 4, the isolated cells induced to differentiate into
hepatocyte-like cells by incubation in basal media with cocktail
hepatocyte growth factors for 20 days.
Results:
In vitro
functional characterization of hepatocyte
detectable by PAS staining for glycogen and immunofluorescent
staining for albumin by anti-human albumin with FITC stain.
Conclusion:
HU-MSC can differentiate into functional
hepatocyte like cells & serve as a cell source for tissue
engineering and cell therapy for hepatic tissues.
e:
mervatdaood@yahoo.comBiomed Res, Volume 30
DOI: 10.4066/biomedicalresearch-C1-026