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Journal of Biomedical Research | ISSN: 0976-1683 | Volume 30
March 14-15, 2019 | London, UK
T issue Engineer ing, Stem Cel ls and Regenerat ive Medicine
Cel l and Gene Therapy
World Congress on
International Conference on
&
Joint Event
Free floating brain sections: An advanced and accurate tool for pre-clinical efficacy histological
evaluation
Emmanuel Loeb
Patho-Logica Ltd., Israel
“Free floating sections” is regarded as a novel histological
method that can be used for immune fluorescence staining.
This method is clearly the most qualitative tool for optimal Ab
expression in the tissue. The usage of the method is changing
the position of histology in pre-clinical trials fromamostly safety
contex, to a potent tool for the efficacy studies. This is the result
of a high quality of the immunohistochemistry cross sections
and the accuracy of morphometric quantification tools that are
often combined with this advanced method. This presentation
covers the technical work pattern of the method starting
with the tissue preparation and conservation, threw brain
accurate dissection and staining. The method is very suitable
for morphometry quantification of histological data, here
method of image analysis will be presented and the scientific
value will be discussed. Furthermore, examples are presented
of projects that had combined the method such as ALS, stroke
and Parkinson models in lab animals. Finally a discussion will
be presented were the advantages of the current method will
be pointed compared to the classical immunohistochemistry
methods.
e:
e.loeb@patho-logica.comBiomed Res, Volume 30
DOI: 10.4066/biomedicalresearch-C1-026