neuro-primary-mental-health-2017-posters-abstracts

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allied

academies

International Conference on

NEUROLOGY AND NEUROSCIENCE

MENTAL HEALTH AND PRIMARY CARE

October 16-18, 2017 | Toronto, Canada

J Neurol Neurorehabil Res 2017 | Volume 2 Issue 3

bnormal tau aggregation is a pathological hallmark

in multiple neurodegenerative diseases collectively

called tauopathies. Mounting evidences suggest that tau

aggregates are not only neurotoxic, but also propagate

in neurons acting as a seed for native tau aggregation.

Accordingly, prevention of prion-like tau aggregation

becomes an important therapeutic strategy to cure the

disease. However, progress has been slow due to the lack

of reliable methods to investigate tau pathology. In this

regard, we developed a cell-based sensor that could monitor

and quantify tau aggregation in neurons. By introducing

bimolecular fluorescence complementation (BiFC) technique

to tau, we could achieve spatial and temporal resolution

of tau-tau interactions in a range of states, from soluble

dimers to large aggregates. Furthermore, by generating tau-

BiFC mouse model that expresses neuron-specific tau-BiFC

expression in the brain, we could visualize tau aggregation

occurs in the diverse brain regions in the brain. Our tau-

BiFC mouse started to present increased BiFC fluorescence

indicating abnormal tau aggregation in the hippocampus

from 6-month-old, and in the cortices from 12-month-old.

Tau-BiFC responses were significantly increased in diverse

brain regions in an age-dependent manner, demonstrating

progression of tau pathology in the brain.

Tau-BiFC platform to investigate pathological tau aggregation

in vitro

and

in vivo

Yun Kyung Kim

Korea Institute of Science and Technology, Korea