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Biol Med Case Rep 2017 | Volume 1 Issue 2
November 06-07, 2017 | New Orleans, USA
Nanomedicine & Healthcare
Global Meet on
Enhancement of antibacterial activity of ciprofloxacin hydrochloride by complexation with sodium cholate
Ugochukwu Jane I
1
, Osonwa Uduma E
1
, Ajaegbu Eze E
1
, Chukwu Kingsley I
2
, Esimone Charles O
1
and
Azevedo Ricardo B
3
1
Nnamdi Azikiwe University, Nigeria
2
University of Saskatchewan, Canada
3
University of Brasilia, Brazil
C
iprofloxacin is a broad spectrum bactericidal anti-
infective agent of the fluoroquinolones class used in
treatment of many bacterial infections. In recent times,
there has been increasing resistance to the antibiotic. In
this work, we investigated the effect of making an ion- pair
complex of ciprofloxacin-hydrochloride with sodium cholate
on bacterial activity. The optimal ratio of the reactants
and pH were determined using UV spectrometry. The
complex was characterized by octanol-water partitioning,
melting point, and IR spectrometry. The antibacterial
activity of the complex was determined against
Escherichia
coli, Staphylococcus aureus, Klebsiella pneumoniae,
and
Streptococcus pneumoniae
by the agar-well diffusion
method. The complex was whitish to off-white in color and
crystalline, with a melting point of 238
0
C. The stoichiometry
of the complex shows a molar ratio of 1:1 of sodium cholate
to ciprofloxacin. The best pH for complexation was pH 9.
The complex partitioned 3.38 times into octanol than in
water. The FTIR revealed interaction between the 4-nitrogen
atom in the 7-piperazinyl group of ciprofloxacin and the
carbonyl of the cholate. The drug in complex form gave
double the antibacterial activity of the uncomplexed drug.
This study showed that development of hydrophobic ion
pair complex enhances antibacterial activity of ciprofloxacin
hydrochloride.
Speaker Biography
Ugochkwu Jane I is a Lecturer in Pharmaceutical Sciences at the Enugu State University
of Science and Technology, Enugu, Nigeria. Her speciality is in Microbiology and
Biotechnology. She is a young dynamic Researcher. She has published many articles in
both local and international journals.
e:
ugoij@yahoo.com