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Journal of Biomedical Research | Volume: 29
November 19-20, 2018 | Paris, France
Molecular Biology, Tissue Science and Regenerative Medicine
International Conference on
Joint Event
&
4
th
World Heart Congress
Exogenous hydrogen sulfide ameliorates high glucose-induced myocardial injury & inflammation via
the CIRP-MAPK signaling pathway in H9c2 cardiac cells
Xiehui Chen
Sul Yat-Sen University of Medical Sciences, China
Aims:
Hydrogen sulfide (H
2
S) is a novel signaling molecule with
potent cytoprotective actions. In this study, we hypothesize that
exogenous H
2
S may protect cardiac cells against high glucose
(HG)-induced myocardial injury and inflammation with the
involvement of the CIRP-MAPK signaling pathway.
MainMethods:
H9c2cardiaccells culturedunderHGconditions
were transfected with siRNA and different inhibitors for
detecting the effects of sodium hydrogen sulfide (NaHS) (a H
2
S
donor) on cell biological processes. The cardiac cell viability
and LDH activity were determined by CCK-8 and LDH kit. ELISA
was employed to measure the levels of inflammatory factors,
while2’,7’-dichlorofluoresceindiacetate (DCFH-DA) toevaluate
reactive oxygen species (ROS). Mitochondrial membrane
potential (MMP) was identified by rhodamine 123 staining.
TUNEL staining and Hoechst 33258 staining were employed
to observe cardiac cell apoptosis. Besides, we determined the
expression of CIRP-MAPK signaling pathway- and apoptosis-
related factors by protein immunoblot analysis.
Key Findings:
HG culturing induced toxicity, LDH, higher level of
inflammatory factors, ROS, MMP, and apoptosis in cardiac cells,
attenuated the viability of cardiac cells, and activated the CIRP-
MAPK signaling pathway. Notably, CIRP silencing aggravated
the above condition. H
2
S or blockade of the MAPK signaling
pathway reversed the above conditions induced by HG.
Significance:
The present study provides evidence for the
protective effect of exogenous H
2
S on HG-induced myocardial
injury and inflammation in H9c2 cardiac cells and suggests that
the activation of CIRP-MAPK signaling pathway might be one of
the mechanisms underlying the protective effect of H
2
S.
e:
Xhchen66@126.comMolecular Biology & Heart Congress 2018, Volume 29
DOI: 10.4066/biomedicalresearch-C8-023