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Journal of Biomedical Research | Volume: 29
November 19-20, 2018 | Paris, France
Molecular Biology, Tissue Science and Regenerative Medicine
International Conference on
Joint Event
&
4
th
World Heart Congress
Beneficial effects of adipose-derived-mesenchymal stem cells (ad-mscs) versus anti-parkinson drug in
a rat model of parkinson’s disease: Relationship to the molecular genetic expressions, ultrastructural
and physiological responses
Naglaa K Idriss
Assiut University, Egypt
Backgrounsd:
Parkinson’s disease is the most common chronic
progressiveneurodegenerativedisorderafterAlzheimer’sdisease.
Theeffectivenessofanti-Parkinsontreatmentsgraduallydiminished
by the progressive degeneration of the dopaminergic terminals.
The current research investigated the effect of adipose-derived
mesenchymal stem cells (AD-MSCs) versus anti-Parkinson drug
in a Parkinsonismratmodel.
Methods:
Forty adult rats divided into 4 equal groups; Group I;
Control group received the vehicle. Group II; Parkinson’s disease
group, received rotenone 2mg/kg daily intraperitoneally for
one month. Group III received rotenone at the same previous
dose then received isolated AD-MSCs on day 14. Group IV
received rotenone at the same previous dose then received
carbidopa/levodopa on day 14. Behavioral tests were carried
out and midbrain specimens were processed for light and
electron microscopy. Genetic expression of glial fibrillary
acidic protein (GFAP) and nestin mRNA were assessed by real
time-PCR. Lamin-B1 and vimentin genes were detected by
gel electrophoresis and plasma levels of angiopoietin-2 and
dopamine were measured by ELISA.
Results:
Rotenoneinducedpronouncedmotordeficits,neuronal
and glial alterations AD-MSCs group showed improvements in
themotor functionandmicroscopicpicture. Foldchangeofboth
genes (GFAP and Nestin) were decreased significantly in AD-
MSC and carbidopa/levodopa group compared to parkinson’s
disease. Lamin-B1and vimentin genes were highly expressed
in parkinson’s disease. Plasma levels of angiopoietin-2 and
dopamineweresignificantlyincreasedaftertreatment(P<0.001)
compared to parkinson’s disease.
Conclusions:
Adipose-derived-mesenchymalstemcells reduced
neuronal degenerationmoreefficiently than theanti-parkinson
drug did in a parkinsonism rat model.
e:
naglaaidriss@hotmail.comMolecular Biology & Heart Congress 2018, Volume 29
DOI: 10.4066/biomedicalresearch-C8-023