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Journal of Systems Biology & Proteome Research
|
Volume 2
J u n e 2 5 - 2 7 , 2 0 1 8 | D u b l i n , I r e l a n d
MASS SPECTROMETRY
AND PROTEOMICS
International Conference on
Margaret Simonian, J Syst Biol Proteome Res 2018, Volume 2
IDENTIFICATION OF PROTEIN
TARGETS IN CEREBRAL ENDOTHELIAL
CELLS FOR BRAIN ARTERIOVENOUS
MALFORMATION (AVMS) MOLECULAR
THERAPIES
Margaret Simonian
University of California Los Angeles (UCLA), USA
Macquarie University Sydney, Australia
T
o develop a new molecular targeted treatment for brain (AVMs),
identification of membrane proteins that are localised on the AVM
endothelium is crucial. Current treatment methods are surgery and
radiosurgery. However, complete occlusion post radiosurgery are achieved
within 3 years, while patient remain at risk of haemorrhage. This study
aims to identify potential protein targets in AVM endothelial cells that
discriminate these vessels from normal vessels; these proteins targets will
be investigated for the molecular therapy of brain AVMs to promote rapid
thrombosis after radiosurgery. We employed
in vitro
and
in vivo
biotinylation
that we developed, and mass spectrometry to detect cell surface-exposed
proteins in cultures of murine cerebral endothelial cells (bEnd.3) and the rat
model of AVM. Two forms of mass spectrometry were applied (iTRAQ-MS
and MS
E
) to identify and quantify membrane protein expression at various
time-points following irradiation which simulates a radiosurgical treatment
approach. Immunocytochemistry was used to confirm the expression of
selected membrane proteins. ProteinPilot V4.0 software was used to analyse
the iTRAQ-MS data and the MS
E
data was analysed using ProteinLynx Global
Server (PLGS) version 2.5 software.
Margaret Simonian has PhD in Advanced Med-
icine and MPhil in Biological Sciences from
Macquarie University- Australia. She works as
a Researcher at UCLA David Geffen School of
Medicine, and previously as a Senior Research
Fellow at LA-Biomedical Research Institute at
Harbor-UCLA, and at Macquarie University. Her
research interests focuses on utilizing Proteom-
ics and Molecular Biology in biomarker discov-
ery and drug development of diseases, such
as brain arteriovenous malformations (AVMs),
brain tumors , aneurysms and multiple sclero-
sis. Her research on brain AVMs was the first
to utilise proteomics to identity protein targets
of AVM molecular and vascular therapies post
radiosurgrey. She presented her research in
many international conferences and published
in many peer reviewed journals. She is also a re-
viewer for the Journal of Proteomics, Journal of
Arthritis & Research Therapy and Journal of Eu-
ropean Proteomics. An Associate Editor for the
Journal of Applied Biotechnology and Bioengi-
neering, and Editorial Board Member for Journal
of Data Mining in Genomics & Proteomics, and
Journal of Science publications, as well as an
Organizing Committee Member for the Interna-
tional Conference on Precision Medicine 2017.
margaret@chem.ucla.eduBIOGRAPHY