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Journal of Systems Biology & Proteome Research | Volume 2
J u n e 2 5 - 2 7 , 2 0 1 8 | D u b l i n , I r e l a n d
MASS SPECTROMETRY
AND PROTEOMICS
International Conference on
Xiaohu Ren et al., J Syst Biol Proteome Res 2018, Volume 2
SET REDUCTION BY MIR-199B-5P
ATTENUATES TRICHLOROETHYLENE-
INDUCED HEPATOCYTE APOPTOSIS
Xiaohu Ren, Jiawen Ruan, Zhihong Chen, Nuanyuan luo and
Jianjun Liu
Shenzhen Center for Disease Control and Prevention, China
Trichloroethylene (TCE) is a commonly used industrial solvent and
widespread environmental pollutant, exposure to which can cause severe
liver damage. Previously we have observed in a model of TCE-induced
apoptosis of cultured human hepatocytes (L-02 cell line) that SET (a protein
encoded by the SET gene in humans) is abnormally elevated which acts as a
key mediator of the induction of apoptosis, however, the potential molecular
mechanisms modulating SET in that model remain unclear. In this study,
through a screening of various databases six microRNAs were predicted to
potentially regulate SET. Subsequent experiment indicated that three (miR-
199b, miR-21, and miR-23a) of them were decreased in TCE-treated L-02
cells. Further analysis using a dual luciferase reporter assay and miR-199b-
5p knockdown/overexpression in L-02 cells revealed that only miR-199b-5p
could suppress SET, through direct binding to its 3’-UTR. Functional studies
indicated that miR-199b-5p attenuated TCE-induced apoptosis of L-02 cells
through the inhibition of SET. In summary, the present study suggests that
in TCE-induced cytotoxicity in cultured hepatocytes miR-199b-5p may down-
regulate SET, thus further attenuating the toxic response to TCE.
Xiaohu Ren has completed his PhD in 2015
from Southern Medical University, and now,
he is post-doctor of Key Laboratory of Modern
Toxicology of Shenzhen. His major research
interests include molecular mechanisms of en-
vironmental chemical-induced liver injury, pro-
teomic profiling of potential biomarkers for the
diagnosis of environmental chemical exposure.
He has published more than 20 research pa-
pers. Additionally, in the past few years, he has
received over six grants, such as Science and
Technology Plan Projects of Guangdong, Na-
tional Science Foundation for Young Scholars
of China, National Science Foundation of China,
and so on.
BIOGRAPHY