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Journal of Systems Biology & Proteome Research | Volume 2
J u n e 2 5 - 2 7 , 2 0 1 8 | D u b l i n , I r e l a n d
MASS SPECTROMETRY
AND PROTEOMICS
International Conference on
Krylov V A, J Syst Biol Proteome Res 2018, Volume 2
GAS CHROMATOGRAPHIC-MASS
SPECTROMETRIC DETERMINATION
OF O-PHTHALIC ACID ESTERS IN
LOW ALCOHOL WINES COUPLED
WITH EMULSION LIQUID-
PHASE MICROEXTRACTION
PRECONCENTRATION
Krylov V A
N. I. Lobachevskii Nizhny Novgorod State University, Russia
Esters of o-phthalic acid are very dangerous for human health. In this
study the high sensitive gas chromatographic-mass spectrometric
determination of phthalates in low alcoholic beverages (champagne, red and
white wine) coupled ultrasound-assisted emulsification-microextraction was
developed. The sources of possible systematic errors were investigated:
Leaking of o-phthalates from chromatographic septum; contamination
of phthalate in solvents; influence of macro components of wines (sugar,
alcohol, anthocyanins); the hydrolysis of o-phthalates and others. For the first
time it is shown that the impact of these factors can lead to an overestimation
or underestimation of the actual concentration of impurities by 1-2 orders
of magnitude. The methods of accounting or elimination of systematic
errors are proposed. Purification of solvents by Rayleigh distillation method
allows to obtain samples with impurity content lower than (1-4).10-3 mgL-1.
Containers for sampling and storage of samples to be analyzed should be
made of borosilicate glass or quartz. The content of o-phthalates in wines
was 0.03 - 1 mgL-1. The limits of detection of esters of о-phthalic acid are
at the level of 10-6–10-5 mgL-1 and are highly competitive with the best world
results. The relative expanded uncertainty of the determination of toxicants
is at the level of 13- 30%.
Krylov V A, Doctor of Chemistry, Professor,
Head of the Division of Analytical Chemistry of
the Nizhny Novgorod State University. The main
direction of scientific research of professor Kry-
lov is the development of the theory and appli-
cations of chromatography for the analysis of
high purity substances, environmental objects
and development of methods of the microex-
traction. The attained detection limits for molec-
ular impurities constitute 10–6 to 10–11 wt % and
hit a record low. He is the author of more than
200 scientific papers, including reviews on the
analytical chemistry of high purity volatile sub-
stances, air and liquid-liquid microextraction
preconcentration.
BIOGRAPHY