3 / 19
allied
academies
Page 21
Journal of Systems Biology & Proteome Research
|
Volume 2
J u n e 2 5 - 2 7 , 2 0 1 8 | D u b l i n , I r e l a n d
MASS SPECTROMETRY
AND PROTEOMICS
International Conference on
Lisa Elviri et al., J Syst Biol Proteome Res 2018, Volume 2
STUDY OF THE INTERACTION OF
ACTIVE COMPOUNDS WITH 3D
PRINTED CHITOSAN/HYALURONIC
ACID SCAFFOLDS IN CELL CULTURE
MEDIUM: A MASS SPECTROMETRY
APPROACH
Lisa Elviri, Ragaiolo M, Bergonzi C, Bianchera A
and
Bettini R
University of Parma, Italy
O
ne of the purposes of tissue engineering is that of developing synthetic or
naturally-derived biological substitutes (scaffolds) capable to help injured
tissues to heal properly. Polymeric materials are often selected as promising
candidates for scaffolding thanks to their high surface-to-volume ratio, their
structural similarity to the matrix and in function of their final biomedical
purpose. Furthermore, 3D biomaterial manufacturing strategies show an
extraordinary driving force for the development of innovative therapies in
the tissue engineering field. Here, the behavior of 3D printed chitosan (CH)
or CH/hyaluronic acid-based scaffolds was explored in terms of mechanical,
morphological properties, and adsorbing properties of low molecular weight
molecules and proteins contained in Dulbecco’s modified medium High
Glucose (DMEM) and bovine fetal serum (FBS), as a function of the gelation
process. Scaffolds were made by a home-made 3D cryo-printing process
from formulations with different concentrations of chitosan and chitosan
and hyaluronic acid, gelled in 1.5 M potassium hydroxide, 1.5 M sodium
carbonate or 28% w / v ammonia vapors. The water content of the scaffolds
together with their mechanical strength and SEMmorphological analyzes were
evaluated. Finally, absorption tests were performed in order to qualitatively and
qualitatively evaluate which substances the scaffold absorbs from the fetal
bovine serumand themediumDMEMHigh Glucose. The analysis conducted by
triple quadrupole and high resolutionOrbitrapmass spectrometry, revealed that
the scaffolds are able to absorb biological molecules present in medium and
serum, and electrostatic interactions are the main driving forces. Furthermore,
molecules presenting an aromatic ring or a sulfur group exhibited a preferred
interaction pathway with the CH/HA scaffolds. The results as a function of the
scaffold properties were presented and discussed.
Lisa Elviri has completed her PhD in 2001 from
Parma University, IT. She is associate profes-
sor of analytical chemistry at the Food and
Drug Department of the University of Parma.
She work mainly on sample preparation, liquid
chromatography, mass spectrometry based
techniques, 3D printing and biomaterial for re-
generative medicine. She has over 90 publica-
tions that have been cited over 2100 times, and
her publication H-index is 26. She is the found-
er and president of M3datek Srl an innovative
start-up dedicated to the 3D printing of bioma-
terial-based medical devices for regenerative
medicine.
lisa.elviri@unipr.itBIOGRAPHY