mass-spectrometry-congress-2018-scientifictracks

allied

academies

Page 36

Note:

Journal of Systems Biology & Proteome Research

Volume 2

J u n e 2 5 - 2 7 , 2 0 1 8 | D u b l i n , I r e l a n d

MASS SPECTROMETRY

AND PROTEOMICS

International Conference on

Nazariy Souchelnytskyi et al, J Syst Biol Proteome Res 2018, Volume 2

AUTOMATION OF SAMPLE

PREPARATION FOR MASS

SPECTROMETRY IN BIOMEDICAL

PROJECTS

Nazariy Souchelnytskyi

and

Serhiy Souchelnytskyi

Qatar University, Qatar

nalysis of separated full-length proteins may be more informative as

compared to a shotgun peptidomics approach. However, automation of

separation and identification of proteins is a serious challenge. When LC-MS/

MS approach is well-automated, 2DE and MS/MS demands a lot of hands-

on work. We will present our experience in development of automation for

sample preparation for MALDI TOF mass spectrometry. Examples will show

applications of the sample preparation robotics in studies of protein nitration,

phosphorylation and expression in human cancer cells and breast tumor

biopsies. Separation of proteins was performed by 2D gel electrophoresis, and

for sample preparation were used liquid handling stations. By incorporation

in the proteomics workflow of robotics stations, we enhanced capacity

of sample preparation in at least 10 folds. Tecan and Genomics Solution

robotics were used, and will be presented as examples of technical solutions.

Nazariy Souchelnytskyi studied at the Royal

Institute of Technology (Stockholm), Uppsala

University and Nakademin (all in Sweden). Mr.

Nazariy Souchelnytskyi has been working as

a proteomics core facility manager at the Lud-

wig Institute for Cancer Research (Uppsala,

Sweden), Karolinska Institutet and Karolinska

University Hospital (Stockholm, Sweden). Mr.

Nazariy Souchelnytskyi is a Chief Technology

Officer at Oranta CancerDiagnostic AB (Swe-

den). He has 9 publications in the application of

proteomics and mass spectrometry in biomed-

ical research.

BIOGRAPHY