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Journal of Biotechnology and Phytochemistry| Volume: 2
October 25-26, 2018 | Frankfurt, Germany
Joint Event
Biotechnology & Medical Microbiology
World Congress on 3 rd International Conference on
Food Science & Technology
Tissue culture propagation in Banana cv. Rajapuri Bale (Musa spp., AAB group)
Prabhuling Guranna, Rashmi H, Kulapathi H, Babu AG and Satish D
University of Horticulture Sciences, India
Banana (Musa spp.) cv. Rajapuri Bale (AAB) is a popular
cultivar of banana grown in Northern parts of Karnataka
state in India. The demands for the planting material of this
cultivar is high and tissue cultured plantlets are not available
because of the inherent problem in the initial establishment of
the culture and multiplication rate is also low. For facilitating
large scale multiplication of this cultivar, the present
investigation was carried out using different antioxidants
and cytokinins to enhance shoot proliferation. The shoot tip
explants were cultured on MS basal medium supplemented
withdifferent kinds and concentrations of antioxidants (ascorbic
acid, citric acid, activated charcoal and dark incubation).
Among the different treatments, ascorbic acid at 225 mg/l
resulted effective control of browning (0 - no browning) and
highest aseptic culture establishment (40 %). However, least
polyphenol oxidase activity (39.55 unit/ml/min) and days taken
for bud sprouting (21 days) were recorded with dark incubation
followed by ascorbic acid (225 mg/l). Multiple shoot clumps
explants inoculated onMS basal medium fortifiedwith different
kinds and concentrations of cytokinins (6-Benzylaminopurine
(BAP), Thiadiazuron (TDZ) and BAP with α- Naphthaleneacetic
acid (NAA). Among different cytokinins, BAP 4 mg/l was found
best for shoot growth parameters like regeneration (100 %),
days taken for bud sprouting (5.41), number of shoots per
explants (3.13), number of leaves per shoot (2.07), shoot
length (4.72 cm) and fresh weight of the plantlets (3374.80
mg/plantlet).The results obtained using RAPD markers showed
94.08 % monomorphism and 5.92 % polymorphism which
was under the permissible limit. The exposure of banana
cultures during in vitro culture to different concentrations of
antioxidants and cytokinins was found not associated with
the risk of genetic instability as revealed by RAPD markers.
Speaker Biography
Prabhuling Guranna has completed his PhD in Horticulture with specialization
in banana plant tissue culture in 2011 from University of Agricultural Sciences,
Bangalore, India. He participated in post graduate course on “Adapting to Climate
Change: Biotechnology in Agriculture in a World of Global Environmental Changes”
from 2.05.2011to 30.06.2011 at Rehovot, Israel. Presently he is working as Associate
Professor of plant biotechnology at University of Horticultural Sciences, Bagalkot,
India. He has over 35 research publications that have been cited over 12 times, his
RG score is 9.11 and H-index is 2 and has been serving as an editorial board member
of reputed Journals viz., Research Journal of Biotechnology and European Journal
of Medicinal Plants. He is MASHAV alumni, life member of International Society of
Biotechnology, Karnataka Horticultural Society and Association for the Improvement
in Production and Utilization of Banana. He received first best oral presentation award
at National Conference on Production of Quality Seeds and Planting Material – Health
Management in Horticultural Crops in 2010.
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