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Journal of Biotechnology and Phytochemistry| Volume: 2
October 25-26, 2018 | Frankfurt, Germany
Joint Event
Biotechnology & Medical Microbiology
World Congress on
3
rd
International Conference on
Food Science & Technology
Induction of disease resistance in regionally important banana cultivars through
in vitro
mutagenesis
Prabhuling Guranna, Rashmi H, Kulapathi H, Babu AG
and
Satish D
University of Horticulture Sciences, India
B
ananas and plantains (
Musa spp
.) represent one of themost
important tropical fruit crops in theworld. It is widely grown
in India with great socio-economic significance. Conventional
breeding methods have been of limited success due to
parthenocarpy, polyploidy and limited available information
on genetics and genomics. Therefore, mutation breeding
combined with
in vitro
culture is regarded as a new technology
for induction of disease resistance in banana. The prime
objective of the present study was to induce disease resistance
in regionally important bananacultivars viz., Rajapuri Bale (AAB),
Ney Poovan (AB), Nanjanagudu Rasabale (AAB) and Red Banana
(AAA) through
in vitro
mutagenesis and screen the putative
mutants against panama wilt and yellow sigatoka diseases. The
multiple shoot bud aseptic cultures were treated with gamma
irradiation (25, 30, 35,40 and 45), EMS (0.30, 0.60 and 0.90 %),
Sodium azide (0.01, 0.02 and 0.03 %) and BAP (10,15 and 20
mg/l). After treatments, cultures were immediately transferred
to multiplication medium. The proliferated cultures were sub
cultured for five times onto fresh multiplication medium with
each subculture involving 25-30 days to disassociate chimeras.
The
in vitro
regenerated putative mutants of Ney Poovan,
Nanjanagudu Rasabale and Rajapuri Bale, Red Banana were
screened for their reaction to panama wilt and yellow sigatoka
disease, respectively in nursery. These putative mutants
were further characterized by using morphological and SSR
markers. Six and five putative mutants derived through gamma
irradiation were found tolerant to panama wilt in Nanjanagudu
Rasabale and Ney Poovan, respectively, whereas, six and eight
putativemutants obtained through chemical mutagenesis were
reported tolerant to panama wilt in Nanjanagudu Rasabale and
Ney Poovan, respectively. About resistance to yellow sigatoka,
six and five gamma irradiated mutants were found tolerant in
Rajapuri Bale and Red banana, while, seven putative mutants
each derived through chemical mutagenesis were reported
tolerant in Rajapuri bale and Red banana. Morphological
and molecular characterization of putative mutants showed
remarkabledifferencesinvariationswithinfourbananacultivars.
Speaker Biography
Prabhuling Guranna has completed his PhD in Horticulture with specialization in banana
plant tissue culture in 2011 from University of Agricultural Sciences, Bangalore, India. He
participated in post graduate course on “Adapting to Climate Change: Biotechnology in
Agriculture in a World of Global Environmental Changes” from 2.05.2011to 30.06.2011 at
Rehovot, Israel. Presently he is working as Associate Professor of plant biotechnology at
University of Horticultural Sciences, Bagalkot, India. He has over 35 research publications
that have been cited over 12 times, his RG score is 9.11 and H-index is 2 and has been
serving as an editorial board member of reputed Journals viz., Research Journal of
Biotechnology and European Journal of Medicinal Plants. He is MASHAV alumni, life
member of International Society of Biotechnology, Karnataka Horticultural Society and
Association for the Improvement in Production and Utilization of Banana. He received
first best oral presentation award at National Conference on Production of Quality
Seeds and Planting Material – Health Management in Horticultural Crops in 2010.
e:
gprabhuling@gmail.com