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Journal of Biotechnology and Phytochemistry| Volume: 2

October 25-26, 2018 | Frankfurt, Germany

Joint Event

Biotechnology & Medical Microbiology

World Congress on

International Conference on

Food Science & Technology

Genotyping of rotavirus in neonatal calves with acute gastroenteritis in Iraq

Atheer Abdulrazzaq Abdulazeez Aldoori

and

Modher Nagem Abed

University of Baghdad, Iraq

lobally, rotavirus is the common major etiologic agents

of diarrhea in infant, young children and neonatal calves.

It is very important to early diagnose the disease for effective

treatment. The objective of this study was to determine

the prevalence, molecular characteristics, and the effect of

rotavirus strains for severe gastroenteritis in neonatal calves in

five Iraqi governorates (Al-Qadissiya, Babel, Kerbala, Missan,

Wassit). A total of 125-stool specimens were examined, it have

been collected from calves form the period between November

2015 to March 2016. The ages were ranging from 6 to 60

weeks. The specimens were examined using chromatographic

immunoassay, enzyme-linked immunosorbent assay (ELISA)

and Polymerase-chain reaction (PCR). Our results gave us

67 (53.6%) positive by chromatographic immunoassay, 45

(36%) positive by ELISA and 32 (25.6%) positive by PCR.

Genotyping were analyzed by multiplex PCR. Genotype

combination G1P[8] was (30%) followed by G1P[4] (20%),

G3P[4] (20%), G2P[4] (10%), G2P[8] (10%) and G9P[4] (10%).

Such information will not only aid in seeking advocacy for

introducing rotavirus vaccine in national immunization program

in Iraq but will also help in the evaluation of the efficacy of these

vaccines in relation to the rotavirus genotyping circulation.

Speaker Biography

Atheer Abdulrazzaq Abdulazeez Aldoori is a microbiologist working in department

of microbiology, Veterinary medical college, University of Baghdad. He got his BSc in

science college, University of Baghdad in 1984 and worked in Al Mansur factory as a

microbiologist. His got his MSc Bacteriology in Veterinary College and worked as head

of department of bacteriology at Al Razee center for research and diagnostic kits in

Ministry of Industry and Materials. Atheer did his PhD in virology from University of

Almustanseria, department of biology and started publishing his works in journals.