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Journal of Biotechnology and Phytochemistry| Volume: 2

October 25-26, 2018 | Frankfurt, Germany

Joint Event

Biotechnology & Medical Microbiology

World Congress on

3

rd

International Conference on

Food Science & Technology

Quantification of Provitamin A and Vitamin C in

Passiflora alata

Curtis and

Passiflora edulis

f.

edulis

Sims Using HPLC

Juan Manuel Parra Gomez, Jorge L Betancurt, Diego A Montoya, John Ocampo, Diego Lope Hector J Osorio

and

Susana Hernández

National University of Colombia, Colombia

T

heediblefruitspeciesofthegenus

PassifloraL.

areimportant

for their nutritional value, health-promoting components,

and medicinal properties. Nutrient composition studies of the

rare and lesser common

Passiflora

species remain central to

assess their potential health benefits. In this work, pulp extracts

of P.

alata Curtis

(sweet passion fruit), a species from Brazilian,

Colombian and Peruvian Amazon, were analyzed to determine

provitamin A and vitamin C contents using High Performance

Liquid Chromatography (HPLC) and the results were compared

with those obtained for

P. edulis f. edulis

Sims (purple passion

fruit). On a fresh weight (fw) basis,

P. alata

and

P. edulis

f.

edulis

contain 3591 g/100 g and 4346 g/100 g, respectively,

of provitamin A (all- trans-β-carotene). Therefore, the vitamin

A content of

P. alata

measured in retinol activity equivalents

(RAE) is 299.3 g RAE/100 g (5985 IU/100 g) and that of

P. edulis

f. edulis

is 362.2 g RAE/100 g (7243 IU/100 g). The total amount

of vitamin C (acid L- ascorbic and dehydroascorbic acid) found

in

P. alata

was 5.78 mg/100 g and

P. edulis f. edulis

was 19.12

mg/100 g. Our results indicate that consumption of sweet

passion fruit and purple passion fruit could provide meaningful

amounts of vitamin A and vitamin C, which would contribute

to achieve the recommended daily intake for both vitamins.

Speaker Biography

Juan Manuel Parra Gomez is a final year undergraduate student at National University

of Colombia, Colombia, South America. He is expected to receive his Chemical Engineer

degree in september 2019. He joined the bioproducts research group of Susana

Hernandez at the same university in february 2016 to work on the identification

of

Ganoderma

species from Colombia, cultivation of

Pleurotus

mushrooms, and

quantification of vitamin C and provitamin A in

Passiflora

species cultivated in

Colombia, using HPLC. The purpose of the bioproducts research groups is to provide

information that can be applied in biotechnology to solve health and nutrition problems

e:

jmparrag@unal.edu.co