22 / 22
Page 60
allied
academies
Journal of Biotechnology and Phytochemistry| Volume: 2
October 25-26, 2018 | Frankfurt, Germany
Joint Event
Biotechnology & Medical Microbiology
World Congress on
3
rd
International Conference on
Food Science & Technology
Molecular characterization of antibiotic resistant
Escherichia coli
isolates recovered from food samples
and outpatient clinics
Hassan A Hemeg
Taibah University, Kingdom of Saudi Arabia
M
ultidrug-resistant
Escherichia coli
is one of the most
important public health concerns worldwide that can
be transferred through the food of animal origin to human
being causing serious infection. The genetic responsibility of
such resistant genes (plasmids, integrons, and transposons)
can be easily transmitted from the resistant strain to another.
Therefore, the main objectives of the study is the molecular
characterization of the resistant
Escherichia coli
isolates
recovered from food samples and human isolates collected
from outpatient clinics, KSA especially the resistance strains
against aminoglycoside resistance genes which are responsible
for the resistance against gentamicin and the resistance caused
b-lactamases genes. Examination of food samples revealed
120
Escherichia coli
isolates (22.22%) (30 strains O26: K60,28
strains O128: K67, 20 strains O111: K58, 18 strains O126:
K58, 10 strains O55: K59, 9 strains O86: K61 and 5 strains
O157: H7). All the strains were highly resistance to penicillin,
amoxicillin-clavulanic and erythromycin with a percentage
of 100%, while the resistance to gentamicin, ampicillin,
oxytetracycline, chloramphenicol, norfloxacin, trimethoprim,
and nalidixic acid were 83%, 75%, 65.3%, 55.8%, 36.5%, 30.7%
and 26.9% respectively. On the other hand, 59.6% of tested
strains were sensitive to ciprofloxacin. Positive amplification
of 896 bp fragments specific for aacC2 genes were observed
by PCR designated for the detection of the aminoglycoside
resistance genes. Meanwhile, multiplex PCR designed to detect
the ampicillin and amoxicillin-clavulanic acid resistant
E. coli
isolates revealed positive amplification of 516 bp fragments
specific for BlaTEM gene with all the resistant strains to
ampicillin and amoxicillinclavulanicacid. Moreover, positive
amplification of 392 bp fragments specific for BlaSHV resistant
gene were observed with (60.52%) of
E. coli
isolate. While all
the tested strains were negative for amplification of BlaOXA_1.
e:
hasanhemeg@hotmail.com