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Journal of Biotechnology and Phytochemistry| Volume: 2
October 25-26, 2018 | Frankfurt, Germany
Joint Event
Biotechnology & Medical Microbiology
World Congress on 3 rd International Conference on
Food Science & Technology
A polysaccharide isolated from Dictyophora Indusiatapromotes recovery from antibiotic driven
intestinal dysbiosis and improves gut epithelial barrier function in a mouse model
Sadia Kanwal
Dalian Medical University, China
Despite the tremendous biological activity of polysaccharides
from the mushroom Dictyophora indusiata, its role in the
restoration of gut microbiota has not yet been explored. The
present study aimed to investigate whether Dictyophora
Indusiatapromotes polysaccharide (DIP) could modulatethe
recovery of gut microbiota composition and intestinal barrier
function after broad-spectrumantibiotic-driven dysbiosis.
Alteration and restoration in the microbial communities were
elucidatedby the Illumina MiSeq platform. Colon histology,
expression of tight-junction associated proteins,and serum/
tissue endotoxin and cytokine levels were evaluated. Two-week
daily oral administrationof clindamycin and metronidazole
resulted in reduced bacterial diversity and richness,
andperturbed the microbial flora at various taxonomic levels
(altered Firmicutes/Bacteroidetes ratioand increased relative
abundance of harmful flora (Proteobacteria, Enterococcus and
Bacteroides ),whereas DIP administration reversed the dysbiosis
and increased beneficial flora, including Lactobacillaceae (lactic
acid-producing bacteria), and Ruminococaceae (butyrate-
producing bacteria).In addition, it resulted in the reduction
of endotoxemia (through lipopolysaccharides (LPSs))and
pro-inflammatory cytokine (tumor necrosis factor alpha
(TNF-α), interleukin 6 (IL-6), andinterleukin 1β (IL-1β)) levels,
with the increased expression of tight-junction associated
proteins(claudin-1, occludin, and zonula occludens-1). These
findings not only suggested a comprehensiveunderstanding
of the protective effects of a DIP in the restoration of gut
microbiota but alsohighlighted its role in the enhancement of
gut barrier integrity, reduction of inflammation andlowering of
endotoxin levels in mice.
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