20 / 22
Page 58
Notes:
allied
academies
Journal of Biotechnology and Phytochemistry| Volume: 2
October 25-26, 2018 | Frankfurt, Germany
Joint Event
Biotechnology & Medical Microbiology
World Congress on
3
rd
International Conference on
Food Science & Technology
A novel immobilized method for isolation of phage display library-derived scFv antibody specific to
Listeria monocytogenes
Xuan-Hung Nguyen
Toulouse III University, France
W
e developed a novel simple and cost-effective
immobilization method for bio panning of a phage-
display library.Weused Light ExpandedClayAggregate (LECA) as
biomass support matrix to isolate phage display library-derived
scFv antibody specific to
Listeria monocytogenes
, a bacterium
causing serious illness in human and animal. Four rounds of
positive selection against LECA-immobilized
L. monocytogenes
and an additional subtractive panning against
L. innocua
were
performed. This panning scheme in combination with our novel
immobilization method allowed us to isolate the phage clones
bind to
L. monocytogenes
without cross-reactivity toward ten
othernon-
L.monocytogenes
bacteria.Oneoftheselectedphage
clones was able to specifically recognize threemajor pathogenic
serotypes (1/2a, 1/2b and 4b) of
L. monocytogenes
and 11
tested
L. monocytogenes
strains isolated from foods. This scFv
antibody has potential use in development of immunoassay-
based methods for rapid detection of
L. monocytogenes
. In
addition, theLECA-immobilizationmethoddescribedhereoffers
an efficient, simple, and cost-effective bio panning strategy
to isolate specific monoclonal antibodies against any given
species of pathogenic bacteria from phage-display libraries.
e:
xuan-hung.nguyen@inserm.fr