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allied
academies
Journal of Biotechnology and Phytochemistry| Volume: 2
October 25-26, 2018 | Frankfurt, Germany
Joint Event
Biotechnology & Medical Microbiology
World Congress on
3
rd
International Conference on
Food Science & Technology
Enhanced bioH
2
and Poly-hydroxyalkanoates production by a co-culture of
Syntrophomonas wolfei
and
a photoheterotrophic mixed consortium using a dark- fermentation effluent as substrate
Zaira Jovana Vanegas Zuniga
National Polytechnic Institute, Mexico
N
owadays, the pollution by oil-based derivatives such as
gasoline, polyethylene, etc is getting problematic. One
possible way to overcome this issue is by developing alternative
green technologies. The bio-plastics production seems to
be a promising method to reduce the plastics production.
Polyhydroxyalkanoates as the copolymers of PHB and PHV have
similar characteristics of the polyethylenes, therefore many
applications.
Synthrophomonas wolfei
(
S. wolfei
) and some
photoheterotrophic bacteria are able to produce this polymer
from the effluents of organic residues treatment. Moreover,
these microorganisms may produce bioH
2
depending on the
culture conditions. However, the production of biopolymers
based on the bacteria metabolism is nowadays still more
expensive than synthetic production. This condition motivates
the research to optimize the biological process to make it
competitive compared to the regular oil-based method.
The purpose of this study was to develop the syntrophic
consortium composed by
S. wolfei
and a photoheterotrophic
mixed consortium named C-4. This strategy would allow to
improve the simultaneous production of bioH
2
and PHA. The
dark-fermentation effluent was used as substrate during
the photoheterotrophic process. This effluent consists of a
complex mixture of volatile fatty acids including acetic, butyric,
lactic, propionic and some others. The data demonstrated the
syntrophic activity between
S. wolfei
and C-4 based on the
comparison of PHA and H
2
productions from the individual and
co-culture fermentations. The individual cultures showed that
consortium C-4 and
S. wolfei
can use the effluent as a carbon-
nitrogen source.
S. wolfei
produced higher concentration of
bioH
2
but lower PHA production compared with C-4. The co-
culture produced this bioproducts simultaneously, with 25%
PHA and 90 mmol v/v H
2
at 100 and 75 hours respectively.
The profile of volatile fatty acids consumption explained the
interaction between C-4 and
S. wolfei
suggesting a mutualism.
e:
ing.zairavanegas@hotmail.com