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Journal of Biotechnology and Phytochemistry| Volume: 2
October 25-26, 2018 | Frankfurt, Germany
Joint Event
Biotechnology & Medical Microbiology
World Congress on
3
rd
International Conference on
Food Science & Technology
Genome sequence of
Mycobacterium yongonense
RT 955-2015 isolate from a patient misdiagnosed
with multi-drug resistant tuberculosis: First clinical detection in Tanzania
Nicholaus Peter Mnyambwa
School of Life Science and Bioengineering, Tanzania
Background:
Mycobacterium yongonense
is a recently
described novel species belonging to
Mycobacterium
avium
complex, which is the most prevalent aetiology
of
non-tuberculous
mycobacteria
associated
with
pulmonary infections, and poses tuberculosis diagnostic
challenges in high-burden, resource-con- strained settings.
Methods:
Whole genome shotgun sequencing and
comparative microbial genomic analyses were used to
characterize the isolate from a patient diagnosed with
multidrug-resistant tuberculosis (MDR-TB) after relapse.
Results:
Thegenomesequenceofthefirstcaseof
M.yongonense
(
M. yongonense
RT 955-2015) in Tanzania is presented.
Sequence analysis revealed that the RT 955-2015 strain had
a high similarity to
M. yongonense
05-1390(T) (98.74%)
and
Mycobacterium chimaera
DSM 44623(T) (98%). Its 16S
rRNA showed similarity to
Mycobacterium paraintracellulare
KCTC 290849(T) (100%),
Mycobacterium intracellulare
ATCC
13950(T) (100%),
M. chimaera
DSM 44623(T) (99.9%), and
M. yongonense
05-1390(T) (98%). The strain exhibited a
substantially different rpoB sequence to that of
M. yongonense
05-1390 (95.16%), but closely related to that of
M. chimaera
DSM 44623(T) (99.86%),
M. intracellulare
ATCC 13950(T),
(99.53%), and
M. paraintracellulare
KCTC 290849(T) (99.53%).
Conclusions:
In light of the OrthoANI algorithm and
phylogenetic analysis, it was concluded that the isolate
was
M. yongonense
Type II genotype, which is an
indication that the patient was misdiagnosed with
TB/MDR-TB
and
received
inappropriate
treatment.
e:
lodnicho@gmail.com