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Res Rep Gynaecol Obstet 2017 | Volume 1 Issue 4
November 02-03, 2017 | Chicago, USA
Embryology and In vitro Fertilization
World Congress on
Valproic acid improved
in vitro
development of pig cloning embryos but did not improve survival of
cloned pigs to adulthood
Xi-Jun Yin, Jin-Dan Kang, Hai-Ying Zhu, Long Jin
and
Guo Qing
Yanbian University, China
T
he objective was to examine the effects of valproic acid
(VPA), a histone deacetylase inhibitor, on
in vitro
and
in
vivo
development of Wuzhishan miniature pig somatic cell
nuclear transfer (SCNT) embryos. Experiment 1 compared
in
vitro
developmental competence of nuclear transfer embryos
treated with various concentrations of VPA for 24 h. Embryos
treated with 2 mM VPA for 24 h had a greater rate of blastocyst
formation compared with control or embryos treated with
4 or 8 mM VPA (21.5% vs. 10.5%, 12.6%, and 17.2%, P<0.05).
Experiment 2 examined the
in vitro
developmental competence
of nuclear transfer embryos treated with 2 mM VPA for various
intervals after chemical activation. Embryos treated for 24 h had
higher rates of blastocyst formation than the control or those
treated for 4 or 48 h (20.7% vs. 9.2%, 12.1%, and 9.1%, P<0.05).
In experiment 3, an average of 207 (range, 192–216) nuclear
transfer embryos from the VPA-treated group were transferred
to surrogate mothers, resulting in three pregnancies. Two of
the surrogates delivered a total of 11 live piglets. However, for
unknown reasons, nine of 11 piglets in the VPA-treated group
died within 1 to 5 d after birth. Untreated control embryos
(average, 205; range, 179–225) were transferred to four
surrogate mothers resulting in three pregnancies, two of which
delivered a total of 12 live offspring, although four of 12 piglets
in the VPA untreated group died (cause unknown) within 1 to 3
d, whereas eight of the 12 piglets in the VPA-untreated group
survived more than 3 or 4 mo. The average birth weight of the
two litters from the VPA-treated group tended (P<0.05) to be
lower than that from the control groups (551.6 g vs. 675.2 g). In
conclusion, VPA treatment increased the blastocyst formation
rate of SCNT porcine embryos; both VPA-treated and the
untreated clones developed to term, but offspring from VPA-
treated embryos had a lower survival to adulthood than those
from control embryos (18.2% vs. 67.0%; P<0.05).
Speaker Biography
Xi-Jun Yin is working as the Director of Jilin Provincial Transgenic Animal and Embryo
Engineering Laboratory at Yanbian University. His research goal is to increase
reproductive efficiency of swine and to expand the genetic potential present in pig
embryos. Recently, his research team successfully produced myostatin gene knockout
double-muscled adult pigs.
e:
yinxj33@msn.com