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Res Rep Gynaecol Obstet 2017 | Volume 1 Issue 4

November 02-03, 2017 | Chicago, USA

Embryology and In vitro Fertilization

World Congress on

Sperm DNA fragmentation on normozoospermic patients

Jheny Díaz, Láyonal Acosta, Carlos Rivas, Lissett Chiscul, Elmer Chavez

and

Luis Gonzales

Gestar

In Vitro

, Perú

Background:

The aim of the present study was to determinate

the incidenceof spermDNA fragmentation innormozoospermic

patients.

Methods:

Prospective study conducted at the Laboratory of

Andrology ¨Gestar

In Vitro

¨ (Chiclayo – Perú). 495 patients were

analyzed from September 2013 to September 2017. The semen

samples were analyzed acording to the criteria WHO 2010.The

Index Sperm DNA fragmentation (IFA) was determined using

the tets Sperm chromatin Dispersion. For determination on the

median was used SPSS 22 software.

Results:

Thepatients’ ages ranged17 to70years. Themeansand

medianwere 37.73 and 37 years respectively. The patientswere

divided into four groups: <30 years, 30-39 years, 40-50 years

and ≥50 years. We determined an inverse correlation between

the age and volumen (r=-0.134

P

=0.000), progressive motility

(r=-0.136

P

=0.000), normal morphology (r=-0.101

P

=0.008),

vitality (r=-0.167

P

=0.000). We determined a significant positive

correlation between age and IFA (r=0.302

P

=0.000). Patients ≥

45 years showed higher levels of IFA (23.5% vs. 16%

P

=0.000)

compared with patients <45.

Conclusions:

The results indicate that advanced age afectts

semen quality and increases the IFA.

Speaker Biography

Dr Jheny Diaz is a physician in gineco-obstetra, she has specialization in assisted

reproduction. She has worked as an medical assistant at Clinica Servimedicos and

Gestar

In vitro

center. She is a member of Latin American Net of Human Reproduction

and society of Gynecology and Obstetrics in Peru.

e:

carlosantoniorivast@gmail.com