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Res Rep Gynaecol Obstet 2017 | Volume 1 Issue 4
November 02-03, 2017 | Chicago, USA
Embryology and In vitro Fertilization
World Congress on
Extracellular vesicles-the potential for translational research in reproductive sciences
Shlomit Kenigsberg
Juno Fertility, Canada
Discarded biological materials retrieved during IVF
procedures are a precious source of information about
tissues and organs of the reproductive system. These materials
include serum, blood, follicular fluid, granulosa and cumulus
cells, discarded embryos, embryo culture media, seminal fluid,
sperm and testicular tissues. However, in a busy IVF laboratory,
the collection and the processing of these samples for research
purposes is challenging and requires not only skilled scientists
and clinical coordinator, but also the engagement of the clinic
staff-the embryologists, nurses, receptionist and physicians.
Extracellular vesicles (EVs), mainly micro-vesicles and exosomes
are released by cells and tissues and were found in everybody
fluid tested so far including theabove sources. TheseEVs contain
proteins, DNA, and subsets of mRNA, miRNAs and other non-
coding RNAs derived from the parental cells. Due to selective
cargo-loading into the EVs, these ‘bullets of information’ are
potential biomarkers and can also be used for therapeutic
purposes. In the present talk, the author will discuss the current
finding from EVs studies in reproductive-related fluids, as well
as the collection, process, analysis, and storage of ART-related
samples in her laboratory. At the end of this presentation,
participants will be able to understand the basic structure and
function of EVs (exosomes), discuss methods to collect and
isolate exosomes and apply these methods to their research
and current findings from EV’s studies in reproduction.
Speaker Biography
Shlomit Kenigsberg is an Independent Scientific Advisor. Her recent position is the
Director of Scientific Affair in a leading Canadian company for Life-Sciences products,
and a Senior Research Associate at Create Fertility Centre from Toronto Canada. She
obtained her PhD studying DNA-methylation in the human malaria causing agent,
Plasmodium falciparum, graduating in 2001, together with an MBA degree. Her first
position was as a Product Manager for QIAGEN, an international BioTech company.
She then accepted a Post-doctoral fellowship with the Department of Human Genetics
at Ben-Gurion University, where she studied differential gene expression in polycystic
ovarian syndrome (PCOS) using microarray technologies. This unique experience led
her to join Create Fertility Centre in 2006 as a Senior Research Associate to establish
a new reproductive biology research laboratory and basic research program. Her
recent projects focused on isolation and characterization of exosomes from various
body fluids and conditioned media in the IVF lab. The project included downstream
applications such as small RNA qPCRs arrays, NGS technologies, bioinformatics
analysis, and proteomics. Although her specialty is in Ovarian Biology, she was also
involved in mesenchymal-stem cells (MSCs) and andrology related research projects.
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