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Res Rep Gynaecol Obstet 2017 | Volume 1 Issue 4
November 02-03, 2017 | Chicago, USA
Embryology and In vitro Fertilization
World Congress on
Evaluation of
in vitro
fertilization outcomes using interleukin-8 in culture medium of human
preimplantation embryos
Guanyou Huang
Guizhou Medical University, China
C
urrently, the morphological method is mainly used
for selecting embryos which are to be transferred, but
this method is relatively poor for prediction of successful
implantation. In recent years, non-invasive observation
of embryo development has been considered as a better
method of embryo viability assessment. The assessment of
embryo quality and prediction of
in vitro
fertilization (IVF)
outcome with cytokines in the culture media (EM) of human
pre-implantation embryos (HPE) has been explored for years.
Researchers have detected tumor necrosis factor alpha (TNF
alpha) and leukemia inhibitory factor (LIF) in EM of HPE, and
have raised the possibility that LIF could have a function as
a factor required for embryo implantation and that high TNF
alpha concentrations seem to be predictive of implantation
failure. However, we could not find the elevation of TNFα in
the EM of human embryos (D3). In this study, the potential of
interleukin 8 (IL-8) in the EM of HPE have been determined,
and the relationship of the IL-8 with embryo quality and the
outcome of clinical pregnancy has been investigated. The EM
from HPE (D3) of IVF/ICSI patients was collected and luminex
high-throughput protein analysis was used to determine the
contents of cytokines in the samples. The results showed
that in patients with media from transferred embryos being
tested positive for IL-8 (IL-8 positive group), the pregnancy
rate, implantation rate and number of live births per
in
vitro
fertilization (IVF) or intra-cytoplasmic sperm injection
patient (N LBPP) were higher than that in patients with
media being tested negative for IL-8 (IL-8 negative group),
and the positive predict value of the IL-8 for predicting the
chance of pregnancy was 56.86%. Compared with the IL-8
negative group, a higher pregnancy rate was observed in
the IL-8 positive group when the patients received equal
quality embryos. Thus, in the EM from HPE, IL-8 may be an
independent predictor for pre-transfer assessment of the
embryo development potential in IVF patients.
Speaker Biography
Guanyou Huang has his expertise in reproductive immunology and embryo
development potential improving the progress of assisted reproductive technique. He
raises the hypothesis for prediction of embryo developmental potential and pregnancy
based on immune characteristics. He intends to establish a system to assess embryo
quality, namely, establish a new and effective system to assess embryo quality on the
basis of secretory and immune function of corresponding embryo.
e:
guanyouhuang@hotmail.com