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Res Rep Gynaecol Obstet 2017 | Volume 1 Issue 4

November 02-03, 2017 | Chicago, USA

Embryology and In vitro Fertilization

World Congress on

Evaluation of

in vitro

fertilization outcomes using interleukin-8 in culture medium of human

preimplantation embryos

Guanyou Huang

Guizhou Medical University, China

C

urrently, the morphological method is mainly used

for selecting embryos which are to be transferred, but

this method is relatively poor for prediction of successful

implantation. In recent years, non-invasive observation

of embryo development has been considered as a better

method of embryo viability assessment. The assessment of

embryo quality and prediction of

in vitro

fertilization (IVF)

outcome with cytokines in the culture media (EM) of human

pre-implantation embryos (HPE) has been explored for years.

Researchers have detected tumor necrosis factor alpha (TNF

alpha) and leukemia inhibitory factor (LIF) in EM of HPE, and

have raised the possibility that LIF could have a function as

a factor required for embryo implantation and that high TNF

alpha concentrations seem to be predictive of implantation

failure. However, we could not find the elevation of TNFα in

the EM of human embryos (D3). In this study, the potential of

interleukin 8 (IL-8) in the EM of HPE have been determined,

and the relationship of the IL-8 with embryo quality and the

outcome of clinical pregnancy has been investigated. The EM

from HPE (D3) of IVF/ICSI patients was collected and luminex

high-throughput protein analysis was used to determine the

contents of cytokines in the samples. The results showed

that in patients with media from transferred embryos being

tested positive for IL-8 (IL-8 positive group), the pregnancy

rate, implantation rate and number of live births per

in

vitro

fertilization (IVF) or intra-cytoplasmic sperm injection

patient (N LBPP) were higher than that in patients with

media being tested negative for IL-8 (IL-8 negative group),

and the positive predict value of the IL-8 for predicting the

chance of pregnancy was 56.86%. Compared with the IL-8

negative group, a higher pregnancy rate was observed in

the IL-8 positive group when the patients received equal

quality embryos. Thus, in the EM from HPE, IL-8 may be an

independent predictor for pre-transfer assessment of the

embryo development potential in IVF patients.

Speaker Biography

Guanyou Huang has his expertise in reproductive immunology and embryo

development potential improving the progress of assisted reproductive technique. He

raises the hypothesis for prediction of embryo developmental potential and pregnancy

based on immune characteristics. He intends to establish a system to assess embryo

quality, namely, establish a new and effective system to assess embryo quality on the

basis of secretory and immune function of corresponding embryo.

e:

guanyouhuang@hotmail.com