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Asian Journal of Biomedical and Pharmaceutical Sciences | ISSN: 2249-622X | Volume 8
&
Joint Event
Chemistry and Organic Chemistry
Biomedicine & Pharmacotherapy
International Conference on
8th World Congress on
October 22-23, 2018 | Frankfurt, Germany
Recent advances in automatic assessment of the cardiac function in zebrafish larvae
Ramesh R Galigekere
Manipal Institute of Technology, India
Studyingmodel organismswithcardiovascular systembearing
similarities to that of the human, has been a part of research
in medicine and biology. Accordingly, zebrafish is considered
very useful for study in developmental biology, toxicology and
pharmacological testing – due to the similarity in the response
to various drugs, easiness of breeding, transparency (up to a
week after hatching), high throughput, lesser cost involved, in
vivo testing, and reduced bioethical concerns. Heart-rate (HR),
its variability andarrhythmia are threenon-invasivemeasures of
cardiovascular health. Although the heart- beats, of a larva kept
under a microscope, may be counted manually, the process is
tedious. Therefore, there is a significant interest in automating
the process, by using a video-recorder fixed to a microscope.
Althoughmany methods to address the indicated purpose have
been published, they have their own respective limitations.
This talk summarizes the findings of recent research performed
by our group – including Mr. Syam Krishna (PhD student at
the Dept. of BME, MIT, Manipal, currently supported by a
CSIR Senior Research Fellowship, Govt. of India), Dept. of
Biomedical Engg., MIT, Manipal, and Dr. Kiranam Chatti, Dept.
of Biology, Principal Research Scientist, Dr. Reddy’s Institute of
Life Sciences, Hyderabad – on the development of a completely
automatic and robust HR-estimation algorithm to on non-
transgenic larvae. The method works automatically to estimate
an adaptive ROI (irrespective of location and size of the larva,
camera zoom, and image resolution) based on the concept of
“quantity of motion”, followed by independent component
analysis. It works even in the presence of minor motion of the
larvae/platform, including drift. The method has worked on all
the videos we have acquired so far. The ability of the method
to detect certain type of arrhythmia is also demonstrated.
e:
Chemistry and Biomedicine 2018, Volume 8
DOI: 10.4066/2249-622X-C4-012