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allied
academies
Cell Science, Stem Cell Research &
Pharmacological Regenerative Medicine
November 29-30, 2017 | Atlanta, USA
Annual Congress on
Adv cel sci tissue cul 2017 | Volume 1 Issue 2
T
he
Oct3/4
gene, a POU family transcription factor, has
been noted as being specifically expressed in embryonic
stem cells and in tumor cells but not in cells of differentiated
tissues. With the ability to isolate adult human stem cells,
it became possible to test for the expression of
Oct3/4
gene in adult stem cells and to test the stem cell theory
of carcinogenesis. Using antibodies and PCR primers, we
tested human breast, liver, pancreas, kidney, mesenchyme
and gastric stem cells, the cancer cell lines HeLa and MCF-
7 and human, dog and rat tumors for
Oct4
expression. The
results indicate that adult human stem cells, immortalized
non-tumorigenic cells and tumor cells and cell lines, but
not differentiated cells, express Oct4.
Oct4
is expressed in a
few cells found in the basal layer of human skin epidermis.
The data demonstrate that adult stem cells maintain
expression of Oct4, consistent with the stem cell hypothesis
of carcinogenesis.
e:
James.Trosko@hc.msu.eduOct4 expression in adult human stem cells: Evidence in support of the stem cell theory of carcinogenesis
Mei-Hui Tai, Chia-Cheng Chang, L Karl Olson
and
James E Trosko
Michigan State University, USA